Studies On The Differential Expression Of Aphis-resisted Genes In Sorghum

MeIanaphis sacchari Zehntner is one of the influential pests on the Sorghum,which leads to heavy loss of the global output and quality every year.How to prevent and cure sorghum aphis is an urgent problem to be solved.The conventional breeding method can't be able to satisfy the agricultural production on resistance aphis.With the development of molecular biology and biological technique,the combination of conventional breeding with genetic engineering used widely in cultivating the anti-aphis variety have become one of research focus.The objective of the paper was to research Differential Expression of Aphis-resisted Genes using cDNA-AFLP from two sorghum cultivars HN16 and QS induced by aphis,to explain the mechanism of Aphis-resisted at molecular level,to excavate and apply the Aphis-resisted Genes.1.The 23 polymorphic primers were screened between HN16 and QS using cDNA-AFLP analysis among 192 primer,pairs.The polymorphic bands between induced and non-induced HN16 varieties weren't detected.However,the 84 polymorphic fragments were found and the 23 differential non-repeat fragments were cloned and sequenced in QS.2.There is,compared with the control,a differential band found in HN 16 by using the primer pairs E36/M51,which was expressed after 24h induced by aphis in QS.3.The 9 genes related to anti-aphis were identified by using the homological analysis based on BLASTx and BLASTn software in GenBank.The result show that there was 97%(194/199)homology between sequence 1 with AOS gene from Oryza sativa,98% (298/304)homology both sequence 4 and LOX-2 mRNA from Oryza sativa,67%(187/278) both sequence 6 and PDF1.2 from Arabidopsis thaliana.100%(180/180)both Sequence 8 and PAL-1 mRNA from Arabidopsis thaliana.97%(292/298)both Sequence 11 and PR-1.1 from Triticum aestivum.100%(67/67)both Sequence 64 and SOD from Gossypium hirsutum.92%(238/256)both Sequence 69 and ADH from Triticum aestivum.89% (168/187)both Sequence 78 and PEPC gene from Zea maize.94%(115/122)both Sequence 81 and Chitinase from Saccharum officinarum.The relationship of other sequences and the published resistant genes is uncertain,and it is expected to research in the future.