Preliminary Studies On Polygonum Aviculare As Feed Additives

The bacteriostatic effects of water extracts,ethonol extracts and ethyl acetate extracts of Polygonum avicidare were studied by the paper disc method and cup method.In addition,double dilution E-tests were used to ascertain the MIC.Results showed that all the three categories of extracts had bacteriostatic effects.Based on the bacteriostatic experiments,the components of the ethyl acetate extract were analyzed by GC-MS.Results showed that the extract mainly contained pyrogallol(50.83%),erythrit(1.95%), thyminose(2.10%),5-acetyl-barbituric acid(0.32%),syringic acid (7.13%),hexadecanoic acid(8.62%),γ-sitosterol(22.8%),and pyrocatechol(2.09%).Among them,pyrogallol,syringic acid and pyrocatechol possessed bacteriostatic activities,which were the substances responsible for the inhibition of bateria.The fatty acids in P.avicidare were extracted by SFE-CO₂ and analyzed by GC-MS.Seven fatty acids were identified:2 saturated fatty acids,palmitic acid and stearic acid,accounting for 22.75%of the total;5 unsaturated fatty acids,accounting for 24.15%of the total.The content of unsaturated fatty acids was obviously higher than that of saturated ones.In addition,the crude protein and crude fibre contents of P.avicidare were 11.6 and 21.1%,respectively.The above results indicated that Polygonum avicidare could be developed as feed additives.