| Small tail cold sheep has the infectious disease in a breeding farm of Kangle County of Gansu province which as appetite, diarrhea, cough, high fever, abortion and so on, with a part of sheep died. These sheep were diagnosed as Pasteurellosis caused by Pasteurella haemolytica in the light of laboratory isolation and cultivation , biochemistry appraisal,animal vaccination and other experiments. The formaldehyde deactivation vaccine was prepared with Hemolytic Pasteurella strain separated in the laboratory ,meanwhile its security and immunity efficacy were determined for the the different adjuvant vaccines. The results have shown that all indexes of the vaccine may satisfy the demand , and are safe reliable.The immunity effect of the vaccine made from the bee glue fluid as the adjuvant surpasses those of the vaccine taking the Al(OH)3 rubber as adjuvant. The vaccine has the strong immunity protection and efficacy for experimental animal(domestic rabbits).The domestic rabbits expressed the immunity during 14 days after they accept the vaccination vaccine ,and achieves as high as 100% protection ratio to the strong toxic bacteria.The highlest effective prices can reach 1280 after 30 days with aboue 4 month's immunity propection duration. WBC, LYM, MID and GRN of the domestic rabbits increase remarkably after the vaccine was injected ,however the RBC content drops. The optimum seal conditions for enzyme linked immunosorbent assay (ELISA) to determinate specifici IgG are follows: The sealing fluid is PBS contained 5%BSA +2% sucrose; furthermore, the effectivest work concentration of the antigen and the serum is diluted in 1:400 and 1:100 respectively.
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