| his study was designed to identify and characterize genes involved in the hemolytic phenotype of the bovine pathogen, Pasteurella haemolytica A1. A recombinant library of P. haemolytica A1 DNA expressed in Escherichia coli was screened for the presence of hemolytic clones. One plasmid that conferred a hemolytic phenotype, pH2, contained an insert with a 774 bp open reading frame that had strong sequence identity to E. coli fnr, a global transcriptional regulator of genes involved in the conversion from aerobic to anaerobic growth. The homologous P. haemolytica gene, fnrP, is a member of the family of fnr-like regulators that includes hlyX from Actinobacillus pleuropneumoniae and btr from Bordetella pertussis. Like hlyX, fnrP complemented the anaerobic deficiencies of a... |
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