| Salvia splendens Ker- Gawl.belong to labiates form section salvia grass and many years living the herbaceous flower.Conduct and actions green the main one of a pillars that turn the flower,a string of red not only beautify the environment,green turn to set up the park and shape the thoughts and feelings,can but also improve with the environmental protection,monitor air pollution,promote the person's healthy body and increases living quantity.In recent years,It have is to green the important contents with organization,school,factory and family,and have positive function with spiritual civilization developments and domestic material in our country civilization developments.Because of its long generally and all thinner and feeble at biennial,not tidy,often make in greenly turning production a year living the cultivation.The flower produces now the industry adopts dibble more to breed,but sproutlace the rate and not high,only have or so 50%,and the posterity individual difference that breed is bigger, lowering the floral personal status,economic performance and not optimistic.Passing the quick heavy technique in tissue culture can overcome the above weakness.Because many years of educating or the species deteriorates,Salvia splendens Ker-Gawl. appeared the assortment not fresh,plant diseases and insect pests serious and the phenomenon that quality descent,affect seriously its appreciate the value and turn green function.But many times plant much expresses for:The anti- is strong,the assortment is gorgeous and spend the long etc,appreciating the value got the exaltation.We make use of the tissue culture technique proceeded Salvia splendens Ker- Gawl.tissue culture system establishes and its autotetraploid researches that times athleticses grow,the aim is breeds selectively to appreciate the worth high and good species.1 Establish and excellent,turned the quick heavy system in Salvia splendens KerGawl. by tissue cultureSalvia splendens Ker-Gawl.tissue culture of the best plant as to take the stem segment with axillaries bud,The caudex segment with a bud is well in the culture medium 1/2MS+ NAA0.1mg/L +6-BA2.0mg/L + IBA 0.5 mg/L,the bud propagates rate is 150.0%;taking root culture medium is 1/4 MS+NAA 0.5mg/L,Taking root the rate amounts to92.0%,taking root the quantity many,the root is robust.2 It was excellent to turned Salvia splendens Ker-Gawl.chromosomes presses a techniqueShow minute details to Salvia splendens Ker-Gawl.the film producer method of tubes seedling a chromosome that authenticate proceeded the excellent turning, establishment the good chromosome show minute details to authenticate the technique with the procedure,combining availably applied in tetraploid the check of times stub departments out.3 Under the tissue culture term proceeded Salvia splendens Ker- Gawl. autotetraploid induce the technical researchIn culture medium,the autumn waters fairy that increase the certain density vegetable,or soak certain time empress to Salvia splendens Ker-Gawl.plain aquas in fairy in an autumn waters for livening bud through certain density to proceed again to educate,the result expresses,all can induct both Salvia splendens Ker-Gawl.tetraploid of creation,among them,behind a kind of method,soak with0.15%autumn waters fairy alkali first namely,then educates in containing a 150mg/ L autumn waters fairy in culture medium,although Salvia splendens Ker-Gawl.tubes the seedling survives the rate lowest,tetraploid induce the rate tallest,attain 69.2%.4 Passing optimizing the condition of the root tine chromosome micro identify,get 10 the autotetraploid stubsElection to choose 5 root-stocks strong,the big flower,the prosperous of growth tetraploid stubs be used as is the breeding material to further accelerates quick heavy, used for in the field contrast experiment.5 The experiment about Salvia splendens Ker-Gawl.tetraploid department and original diploid plants proceeded to moveSalvia splendens Ker-Gawl.tube transplant is better under the condign wet and temperature term,the result expresses,the live rate can amount to 85.8%above. |
