| Zoysia matrella(L.)Merr.,is widely used as warm-season turfgrass.It has many attractive characteristics,for example,its leaf blade is fine textured and greenish,it has a long-green period and is extremely drought tolerant,it can be grow in all kinds of soils ranging from sands to clays and both salt and alkaline in reaction,it also grows well in shaded locations.Due to its high quality,it is widely cultivated in the south of China and grown as an ornamental grass.It makes ideal lawn grasses in some situations and can be used on playground and athletic fields.It is also ideal for erosion control.However,it also has some defects in real-life application.For instance,it is slow to develop a very dense turf,demonstrates bad cold tolerant and has rust disease.In addition,it turns brown after several hard frosts and remains brown until late spring.So it is necessary that using breeding methods improves its features to be adapted the social development.The integration of conventional breeding methods and modern biology technique,will be able to decrease the period of breeding greatly.The main objective of this study was to study on tissue culture and plant regeneration of Zoysia.matrella which will be valuable for the genetic improvement by genetic engineering means.The main results were as follows:1.Method of obtaining in vitro plantlets:firstly,taking Zoysia matrella stem with buds and the length 1.0～2.0 cm as explants;secondly,sterilizing with 5 g abluent+5%sodium hypochlorite(10 min)+0.1%mercuric chloride(10 min):at last,incubating explants horizontally on the MS medium and culturing with 16 h illumination/8 h dark photoperiod.2.The concentration of IBA(0.1～2.0 mg/L)and NAA(0.1～0.5 mg/L)were favorable for in vitro plantlets to grow and root,respectively.The concentration of 2,4-D(0.1～1.0mg/L)was favorable for in vitro plantlets to grow.The respective addition of 6-BA and KT were helpful to proliferate,but harmful for in vitro plantlets to grow and root.3.The medium which was beneficial to proliferate was MS+IBA 0.1 mg/L+6-BA 0.1 mg/L. The average number of buds were up to 9 after 30 days,when in vitro plantlet was cultured in the medium.4.It was good for inducing callus that taking in vitro plantlet which was thriving as explant and MS+2,4-D 2.0 mg/L+6-BA 0.02 mg/L as medium by culturing with 16 h illumination/8 h dark photoperiod.The ratio of callus induction was up to 70%.5.The medium for callus subculture was MS+2,4-D 1mg/L+6-BA 0.01 mg/L and calli were subcultured 25 d.The best differentiation medium was MS and the ratio of callus differentiation was 14%.6.In vitro plantlet and regenerated plant were easy to root,and the ratios of survivance were up to 90%after in vitro plantlet and regenerated plant were transplanted,respectively. |
