The Some Physiological Characteristics, Microscopic Structure Of Leafs And Protein Characteristics Of Three Species Oleaster Under NaCl Stress

Elaeagnus angustifolia L. is one kind of important economical tree in Xinjiang Uygur Autonomous Region, It has the strong compatibility to the salt and the drought, One-year-olds seedling of E. oxycarpa Schlecht. , E. mooceroftii Wall.ex Schlecht, Elaeagnus angustifolia var orien talis(L.)Kuntze were used in this experiment as materials. The NaCl concentration is 50, 100, 200, 300 and 350mmol/L respectively, salt treated about 10, 20, 30 days in potted mulberry under the rainproof awning, to study systemically the changes of the growth, the water content, the electron microscopic structure of leafs, the POD band and protein change situation. And discussed the research measures of salt-tolerant characters and evaluate salt-tolerant ability of Elaeagnus angustifolia L..Main results as follows:(1) After salt treated about 10, 20, 30 days, with the increasing of the concentration of NaCl, the different organs and whole biomass weight declined, and wet Root-Crown ratio was more than dry Root-Crown ratio, their had different salt-tolerant ability, water potential of leafs decreased, leaf relative water content and succulence index of leafs had changed slightly in E. oxycarpa Schlecht., E.mooceroftii Wall.ex Schlecht, Elaeagnus angustifolia var orien talis(L.) Kuntze, Especially at the strong salt concentration(ï¹¥200mmol/LNaCl), They still could maintain the low water potential, promoted root absorbing water upward transportation, maintains high water potential in leafs, thus causes the plant to carry on the vital activity.(2) Along with the increasing time and degree of salt stress, leaf chlorophyll content, car content and chlorophyll a+b value increased in E. oxycarpa Schlecht. and Elaeagnus angustifolia var orien talis(L.)Kuntze, and decreased in E. mooceroftii Wall.ex Schlecht, chlorophyll a+b value had changed slightly in third. Na+ content had great degree increased. Moreover, in the comparison, 200mmol/L, 350mmol/LNaCl leaf microstructure comparison, three mesophyll cells suffered the degree to increase, the intercellular space filled out, the vacuole changed small, but the chloroplast structure is complete, the grana lamella is clear.(3) When salt stress 30 days, the POD band is 4 in E. oxycarpa Schlecht. and E. mooceroftii Wall.ex Schlecht, but the band color depth is different at each treatment, The protein content of leafs increased, and the quantity changed slightly, protein band was no change in the E. oxycarpa Schlecht., when 300, 350mmol/LNaCl concentration the protein band number increased in E. mooceroftii Wall.ex Schlecht, when 200, 300, 350mmol/L NaCl concentration the protein band number increased Elaeagnus angustifolia var orien talis(L.)Kuntze, whether these additional banding for the salty stress inducted, it will wait for further studies.(4) The results show: Salt-resistance ability of E.oxycarpa Schlecht. and Elaeagnus angustifolia var orien talis(L.)Kuntze are higher than E. mooceroftii Wall.ex Schlecht.. and they have the different salt-resistance mechanism,different indexes have different contributions to salt-resistance of them.plant wet height, plant dry height, Root-Crown ratio, the percentage of dry matter accumulation, organic content in leafs, water potential, leaf relative water content, succulence index of leafs, leaf chlorophyll content, car content and chlorophyll a+b value, chlorophyll a/b value, leaf microstructure, protein content can be used to appraise the capacity of salt-resistance of Elaeagnus angustifolia L. The POD band and protein band in leafs may for be able to appraise better the Elaeagnus angustifolia L. salt-resistance ability.