| Pepper Anthracnose caused by Colletotrichum spp. is one of the most economicallydestructive diseases of pepper(Capsicum annuum L.) throughout the world and can be alimiting factor for pepper production in tropical areas with hot and humid climates. Therefore,screening effective fungicides and antagonistic microbe to Colletotrichum spp. is the mainstudy target for controlling pepper anthracnose.Studied bioassay of Carbendazim, Thiram, Kocide, Mancozeb, Polyoxin B andProchloraz to Colletotrichum capsici, Prochloraz was the best inhibition to Colletotrichumcapsici mycelium growth with an EC50 of 0.84μg/ml. Carbendazim and Thiram could inhibitmycelium growth of Colletotrichum capsici.Strain A and B antagonized to Colletotrichum capsici were isolated from pepper land inHunan Province. The inhibited Colletotrichum capsici mycelium growth percent of diluted 10times from strain A and B fermenting filtrate were 48.9％and 71.8％, respectively. Theinhibited Colletotrichum capsici spore germination percent of diluted 5, 10, 20 times fromStrain A fermenting filtrate were 64.8％, 57.4％and 51.6％, respectively; Strain B, 63.4％,59.4％and 46.9％, respectively. In addition, strain A and B could effectively inhibit myceliumgrowth of Colletotrichum lindemuthianum, Alternaria alternata and CollectrichumIagenarium. According to cultivating characteristic, physiological and biochemical test, strainA and B were elementary identified as Streptomyces spp..The fermenting filtrate of strain A and B could enhance germination and growth ofpepper seeds according to allelopathy research. Cultivating liquids of strain A and B couldcontrol Pepper Anthracnose according to filed test. The control effect of diluted 50 times ofstrain A and B cultivating liquids were 63.6％and 64.26％, respectively; Diluted 200 times,51.54％and 49.93％. It showed that strain A and B were potential bio-control agent to PepperAnthracnose.
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