| The sucrose ester is a kind of important fragrance precursor material in the tobacco,by the gland cilia secretion production. This article take the Yunyan85, K326, K346, G80,NC89, NC82,Hong HuaDaJinYuan, 7 flue-cured tobacco varieties as experiment samples,using the highly effective thin layer chromatograph scanning method to carry onqualitative and the quantitative analysis to the flue-cured tobacco leaf tobacco surfacesucrose ester; Studying different leaf position leaf tobacco surface sucrose ester and otherchemical composition relevance for the different genotype flue-cured tobacco at the sametime; Researching leaf tobacco surface sucrose ester and other chemistry target dynamicchange of flue-cured tobacco in different mature course,At the same time, using themethod of natural rain and tectorial membrane to analyze and compare rain waterinfluence upon the leaf tobacco surface sucrose ester. The main findings are as follows:(1) In order to create the condition for the fast examination and the analysis of leaftobacco surface sucrose ester, adopting thin layer chromatograph scanning analysismethod that using the highly effective thin layer chromatograph scanning methodestablishment separation appraisal leaf tobacco surface sucrose ester. After trying twodifferent flowing direction, discovering that the leaf tobacco sucrose ester separationeffect is good that taking the toluene-ethyl acetate-methyl alcohol-water (volumeproportion is 10: 5:4.5:0.2) as the developing solvent, the urea-phosphoric acid-normalbutyl alcohol mix is the developer. The thin layer chromatography's operation is simple,the sensitivity is quickly high, accurate reliable, the reproduction quality is good, theresult is stable, and it can separate the flue-cured tobacco sucrose ester effectively, and isextremely suitable for the mass sample's analysis, which may be a effective method forthe flue-cured tobacco sucrose ester analysis.(2) At the same time carries on the sample to 7 different genotype flue-curedtobacco after pinching off, determining different genotype roasts the leaf tobacco surfacesucrose ester secretion and analyzing it with other chemistry target relevance. The resultsindicated: Different genotype flue-cured tobacco surface sucrose ester total quantity isobviously different. The difference of various position leaf tobacco surface sucrose estercontent presents the similar change tendency, the NC89 content is highest, next is theYunyan85, the lowest is G80. Comparing the leaf position discovered upside that thevariety of sucrose ester total quantity presents the leaf＞middle the leaf＞lower partleafs rule. The relevant analysis result indicated that the difference of different variety ofthe sucrose ester content is remarkable, but the different leaf position differs from. The sucrose ester and the soluble total sugar, the return to original state sugar and the nicotineare positive correlation, and it is negative correlation with the non-return to original statesugar and the potassium.(3) In the flue-cured tobacco mature process, dynamic change of the leaf tobaccosurface sucrose ester and other chemistry target research indicated that middle leafsurface sucrose ester content in the 0-28d time section assumes the rise basically after tippruning,the tendency, in 28-35d starts to recede slowly after tip prtming; Different varietyin mature process sucrose ester's main change tendency of sugar, return to original statesugar, and nicotine are basic consistent, assumes the opposite change tendency with thepotassium.(4) Using the method of the natural rain the tectorial membrane to research naturerainfall take effect on Hong Hua Da Jing Yuan with the K326 leaf tobacco surfacesucrose ester and other chemistry target total content. The findings indicated that the rainwash has a great influence on the leaf tobacco surface sucrose ester content. Comparingthe leaf position, middle of the leaf receives affects is bigger than the upside leaf.Comparing variety, K326 receives affects must be bigger than HD, and affects most about21 d after pinching off. Rains influences on the sucrose reduced when the later period ofleaf tobacco mature. The influence of rainfall to the soluble total sugar, the return tooriginal state sugar, the non-return to original state sugar, the nicotine and the potassiumcontent is small, the variety difference is not so great. |
