Study On Microorganism Isolated Factors And Microflora Of Rhizosphere Soil Of Pinus Tabulaeformis And Ostryopsis Davidiana

In damaged Daqingshan mountains ecosystem, Ostryopsis davidiana and Pinus tabulaeformis can grow well. The paper studied on microorganism of mycorrhiza of their woodlands. Made a series research on the separating conditions and inhibitors using.Selected several important influencing factors and established new optimal combinations on separating from soil of mycorrhiza base on the traditional method. The result indicated that bacteria separating optimal combination is SEB culture mediumï¹¢Big beading (Φ=5⁷mm ,55grains)ï¹¢no Sodium Deoxycholateï¹¢Vibrating time 90min(160rad·min-1). Fungi separating optimal combination is MA culture mediumï¹¢Big beadingï¹¢1% Sodium Deoxycholateï¹¢Vibrating time 60min. Actionmycetes separating optimal combination is no heat treatment +SGEA culture mediumï¹¢Big beadingï¹¢1%Na4P2O7·10H2Oï¹¢Vibrating time 60min.Experiment testified that tetrachlorofluorescein is not a better inhibitor in fungi separation, Amikacin is a perfect inhibitor in fungi separation. It can restrain the bacterial growing and have no infection in fungi separation, the best concentration is 0.1ml·L^-1. Potassium bichromate is the first choice inhibitor of rhizospheric actionmycetes'separation, the best concentration is 10mg·L-1. But high concentration can inhibit actionmycetes grow, the concentration come to 60mg·L-1, the separate number of actionmycetes decrease evidently. So choose Potassium bichromate as actionmycetes'inhibitor must pay attention to control its concentration.Microorganism biomass of Pure Pinus tabulaeformis and Ostryopsis davidiana and mixed forest are obviously higher than forest edge. Change regularity of rhizospheric microorganism of mycorrhiza in Pure Pinus tabulaeformis and mixed forest are similar in different season, but pure Ostryopsis davidiana changed extent is more observably.Through the form observation we identified fungi 20 genera 55 strains. There are 23 strains haven't identified, most of them have no spore and conceptacle, it's hard to identified by form observation. Through the form observation and physiological and biochemical test we identified bacteria 16 genera 41 strains, 10 strains haven't identified. 8 genera 26 strains of actionmycetes have identified, but 9 strains haven't identified.