| The study was mainly to probe an efficient way through tissue culture and rapid propagation ofSarcococca ruscifolia Stapf. Leaves, buds and mature embryo were taken as explants. The tissue culture and plant regeneration ofSarcococea ruscifolia Stapf were systematically studied. The main results as below:1. The best season of taking explants on Sarcococca ruscifolia Stapf is in spring, the starting rate, the rate of forming callus, surviving rate and growth inducement rate are the highest; the browning rate and contaminated rate are the lowest, especially in April of spring.2. Sucrosecose could rather promote the growth of explants in Sarcococca ruscifolia Stapf, especially 30 g·L-1, the effect of sucrose inducement takes second place, and white sugar is comparatively lower in this experiment.3. CH could rather promote the growth of explants in Sarcococca ruscifolia Stapf, especially 450 mg·L-1, the effect of LH takes second place, but difference of them is not marked in this experiment.4. Explants in Sarcococca ruscifolia Stapf could rather growth in MS culture medium; The growth inducement in 1/2MS culture medium takes second place, and that in B5 culture medium takes third place, and SH culture medium takes the lowest.5. Inducement and cultivation of buds: The optional culture medium combination for start and inducement of buds in Sarcococca ruscifolia Stapfwas: MS+6-BA 1.0+NAA 0.1+IBA 0.05+sucrose 30 g·L-1CH 450 mg·L-1, the inducement rate was 85.3%.6. Callus induction from leaves and mature embryo: The optional culture medium combination for calluses inducement from leaves and mature embryos in Sarcococca ruscifolia Stapfrespectively were: MS+6-BA 1.0+IBA 0.5+2, 4-D 0.1+sucrose 30 g·L-1+CH 450 mg·L(-1), the induction rate could be up to 80.1%; MS+6-BA 1.0+2, 4-D 0.1+sucrose 30 g·L-1+CH 450 mg·L-1。7. Proliferating and differentiating cultivation: The optional culture medium combination for buds proliferation was: MS+ 6-BA 1.0+NAA 0.1+IBA 0.1+sucrose 30 g·L-1+CH 450 mg·L-1, proliferation index was 6.22 after inoculating 28 day; The optional culture medium combination for leaf and embryo calluses proliferation respectively were: MS+6-BA 2.0+NAA 0.5+2, 4-D 0.1+sucrose 30 g·L-1+CH 450 mg·L-1, MS+6-BA 1.0 +NAA 1.0+2, 4-D 0.1+ sucrose 30g·L-1+CH 450 mg·L-1, and the proliferation rate could be up to 77.4%, 66.7%; The optional culture medium combination for leaves calluses differentiation was: MS+6-BA 1.0+NAA 0.5+sucrose 30 g·L-1+CH 450 mg·L-1, the differentiation rate could be up to 75%; The optional culture medium combination for mature embryo differentiation in Sarcococca ruscifolia Stapf was: MS+6-BA 1.0+NAA 0.1 sucrose 30 g·L-1+CH 450 mg·L-1.8. Rooting culture: The optional culture medium combination for inducing buds rooting in Sarcococca ruscifolia Stapfwas: 1/2 MS+ IBA 1.0+NAA 0.1+AC 1.5+sucrose 15 g·L-1, the rooting rate could be up to 70.3%.9. Transplanting: Plantlets were transferred to the medium with ratio of 3: 2: 1 of vermiculite and ruby mica and sands, the survival rate was up to 70.0%.10. Cutting propagation: Cuttings would root in the shortest time, the quantity of roots was the most, the length of root was the longest, and the rooting rate was the highest when cuttings were treated with NAA+IBA with 25.0 mg·L-1 dipping 40 min. |
PDF Full Text Request