Study On Sex Identification In Seedling Stage Of Trichosanthes Kirilowii

Trichosanthes kirilowii belongs to Cucurbitaceae and Trichosanthes.it is trict dioecism plang many uses. The purpose of this paper is to identify sex of seedling Trichosanthes kirilowii. This study is helpful to rational field planting ratio of male to female Trichosanthes kirilowii in orchard for commercial production. In this paper, the identification technique in female and male plant of Trichosanthes kirilowii was studied. By contrasting the difference of dyeing colors by chemical reagents such as BTB, methyl red, and bromophenol blue measuring SOD, EST and PPO activity by gel electrophoresis and AFLP techniques. The following results and conclusions were drawn:(1) The leaves of male and female Trichosanthes kirilowii Maxim. were treated respectively with chemical reagent. The sexes were identified quickly and efficiently through different color and precipitate reaction among them.The difference is significant.(2) Three isoenzymes, SOD, EST and PPO were analyzed by using polyacrylamide gel electrophoresis(PAGE) and biochemical staining in Trichosanthes kirilowii Maxim. The result showed that the electrophoretic patterns of the SOD isoenzymes sex-related differences between male and female in petiole, tendril, leaf and stem tip, whereas the EST electrophoretic patterns showed no difference in both sexes in petiole.(3) First research used AFLP technique to study Trichosanthes kirilowii's sex, and an AFLP technique system suitable for Trichosanthes kirilowiir was established and optimized.433 AFLP bands were amplified in agarose gel electrophoresis by 34 pair primers from in male and female Trichosanthes kirilowii Maxim.16 bands were amplified for each primer pair. There were 71 polymorphic bands between male and female bulks, 40 from male plants and 31 from female plants, the separated rate was about 1.3:1.