Xinjiang Northern Border Partial Intensification Cattle Farm Bovine Viral Diarrhea Virus Molecular Epidemiology Investigation

In order to understand the northern of XINJIANG province Bovine viral diarrhea virus the infection condition, as well as BVDV gene distribution, In the cattle farm gathers the cow from North Xinjiang local cow's blood and rectum mucous membrane. Examine mucous membrane bovine viral diarrhea virus by Standardization of Ag-Capture ELISA Kit for Detecting Bovine viral diarrhea virus. Gathering sample 192 shares, 576, average masculine gender rate is 35.4℅; The sample masculine gender rate is 100℅ in arising cattle farm, looked resembles normal cattle farm picking out rate is 0 - 57.9℅ to be different; The normal cattle farm average masculine gender rate is 23.5℅ .The sample average positive rate is 0– 100℅to be different.According tocow virulent diarrhea virus's standard poisonous 5′-UTR NADL,conservative area design primer, Examines the cow blood with the RT-PCR method the cow virulentdiarrhea virus. The goal piece recycling clone and sequencing。And uses DNAMAN, Primer biological information study software analysis,makes north Xinjiang the local cow's bovine virulent diarrhea virus'ssystem evolution tree。Raise MDBK,Select the masculine sample cowblood after repeatedly the freezing and thawing, after entrifugal processing filters except the fungus turns on is covered withsingle-layer the MDBK cell, after continuously blind biography fourgenerations, judgement gathering sample, selections sample has not sent the cell pathological change,so must sends the cell pathological change (ncp).