| Semen collection of mamal is extensive concerned in science, owning to importancevalue in science and the application.The raccoon dog is differrent with domestic animals inphysiology characteristics, This bring a very great difficulty in the research of semencollection of the raccoon dog.Electroejaculation have been reported a littile in semencollection of the raccoon dog. This research is taking three different ways, Artificial vagina,Electroejaculation, Massage method.Three kinds of methods collect a technique to carry onAI of the raccoon dog, the main contents include:(1)The experiments were involved electroejaculation from 13 healthy raccoon dog whichwere 42 time to semen collection,During that time, the raccoon dog were ejaculated 38 times,which held 90.5 percent to the total number.The ejaculation volume of the raccoon dog was1.52±1.23ml; pH was 6.56±0.37, the sperm concentration was 5.43±1.08 million,Spermmotility was 91±0.2, The integrity rate of acrosome wasl 1.23±0.37%.Electric voltage of ejaculation was 25～40V, Electric current frequency was 40～45Hz, the raccoon dog begin to ejaculate in 11～13min.The whole process is colected in 15～20min.(2)This experiment have designed a Artificial vagina and improved 2 times in sememcollection, based on analysis of mating and the physiological dissection, one AV to collectsemen artificially was established initially.Some new data on the artificial vagina werepresented,one kind of new way to control the temperature has guaranted constantly vacationvagina temperature for long time, using the body temperature and water temperature tocontrol together.The pressure may adjustment freely through the chain ball;this experimenthave been imitated a mating behavior(lock knot) in nature, through having been placeda"lock knot ball" at outer shellof AV. Although this experiment didn't succeed to picked seminalfluid, it is the first research, on it and provide basic technical parameter for the furtherresearch in the AV of the raccoon dog.(3)The experiments were involved Massage method from 57 healthy raccoon dog whichwere 96 time to semen Collection from 2006 to 2007. During that time, the raccoon dog wereejaculated 26 times, which held 27.9 percent to the total number. The ejaculation volumeof the raccoon dog was 0.29±0.18 ml. pH was 6.6±0.19, the sperm concentration was0.99±0.83 billion, Spermmotility was 0.9±0.2, The integrity rate of acrosome was16.41±2.20%,Rate of living was 85.11±8.3%.The experiment analyzed mainly to five aspects in many factors, which effect results ofsemen collection,which includes the stimulated spot, the training, the incentive, the germ cells growth,and the animal selection.The result indicated, main stimulation spot to semination ofraccon, dog is in both sides behind penis bone,in channel place below Glans penisball.Restraining before semen collection from raccoon dog decrease stressTo new environmentand increases matching to holder for 3～4 day.The incentive stimuli are effective in increasingthe sexual response,and increase the number of sperm cells obtained.Considered from thelong-term angle, to enhance the fine success ratio of semen collection,collecting fine raccoondog is essential by sex glands and the breeding.record.Conclusion: This study believed,artificial vagina is not successful in Semen collectionfrom the raccoon dog. The design to Artificial Vagina still had to be improved.Althoughelectroejaculation of the raccoon dog is above 90% in success ratio,it is not suitably in using tothe AI of the raccoon dog,because opratation is complexed,and it injury animalveryquitely.Massage Method is possible to apply in artificial insemination,if we selection fineseed and restraining before semen collection.
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