Study On The System About Tissure Culture And Rapid Propagation Of Catalpa Bungei

Catalpa bungei belonged to Bignoniaceae Catalpa,a deciduous treewhich was origin of our country,was a valuable quality timber speciesand the famous ornamental trees, traditional cultured in China.Catalpa bungei was called "The king of wood" since ancinet times.Catalpa wood's not only had a good physical properties-longitudinal compressive strength,flexural strength,but also had a good chemical properties-corrosion resistance,it could be applied to the High furniture,equipment and special timber.At the same.time,Catalpa was tall trees with deep-roots,substantially increased in regional defense functions.Catalpa tree with hysteresis dust,absorbed harmful gases,such as sulfur dioxide,chlorine and had a strong resistance to other toxic gase.It was clear that improving the ecological environment,raised the city's disaster response and performance.Catalpa bungei was planted for greenning neighborhood and increasing vegetation coverage rate,also as a major green species.and beautified the environment.The axillary buds of the Catalpa bungei pedicels were taken as explants to induce bud clumps and this regeneration way of propagation were studied in this experiment.This experiment resolved around the best ways to exterminate germs of explants,producing cluster buds,propagating cluster buds,strengthening test～tube shoots,engending roots and transplanting finally.The main contents focus on the following aspects:asepsis methods,basic mediums and raising modes,the combination and density of hormone,, carbon source and different resisting oxidizing agent.The Orthogonal Design is adopted to arrange experiments,and all data analysis was dealt with by SPSS.Through the research above,the main effected factors and the suitable medium in each stage were researched fully,then the technique parameter combination were filtrated,the superior regeneration system about Catalpa bungei to expand the choice scope of explants, improve the propagation coefficient,and,thus enhancing Catalpa bungei commercial production efficiency,provide solution to the current market shortage of fine breed Catalpa bungei,and technical support for professional nursery.1.The result shows that the effective procedure of setting up asepsis shoots is follow: the axillary buds of the Catalpa bungei pedicels? washed by tap water? dipped in the detergent water about 10min and used a soft brush to clean the surface dirt? washed about 30min by tap water? putted on the extra-clean desk? dipped in 70%alcohol 8S Cotton to clean? washed 2times by asepsis water? dipped in the 400mg/l Penicillin and the 200mg/l Streptomycin about 20min? dipped in the 0.1%HgCl₂8min? washed 8 times by asepsis water? soaked in 100mg/LPVP,the beginnin and the end cut 1cm individually? water dried and the bracteal leaves sweeped away? inoculated,the success rate is 55%.2.The pollution of shoots may be disinfect is follow:dipped in the 0.25%iodophor 3rain? dipped in the 0.1%HgCl₂8min? washed 8 times by asepsis water? soaked in 100mg/LPVP,the success rate is 30%.3.The significant factors of germination axillary buds is medium,BA,ZT and IBA.The best inducement medium is 1/2MS+BA1.0 mg/L +ZT 0.2mg/L+IBA0.2 mg/L+ sugar 30g/L + agar 8.0g/L,pH5.8.4.The main factors of bud lumps multiplication are the basic medium,BA,IBA,carbon source,agar and the kind of the resisting oxidizing agents.The medium which has pvp(100mg/l)can improve the multiplication of bud clumps.The medium without agar can significantly improve the multiplication of bud clumps.The effect of carbon source is not obvious.The best multiplication medium is MS+IBA0.5 mg/L+BA4.0 mg/L +PVP(100mg/L)+ sugar 30g/L + agar 8.0g/L,pH5.8.5.The significant factors of rooting is medium,NAA,IBA,IAA.The best medium for rooting is WPM+NAA0.2 mg/L+IBA0.6 mg/L+IAA0.1 mg/L+ sugar 30g/L + agar 8.0g/L,pH5.8.Last,we can use the medium of 1/2MS+NAA0.5 mg/L+IBA0.6 mg/L+IAA0.3 mg/L + sugar30g/L + agar 8.0g/L,pH5.8 to increase the number of root and the length of root.If the medium has not agar,impact to rooting is not obvious.but not to wash agar.6.When transplanting,first,seedlings are forged in the room for 6days,then transplanted.Vermiculite is the best soilless media for Catalpa bungei,the living rate can reach 70%,meanwhile,seedlings grow strongly,and leaves glitter and all of them are upstanding.