Effects Of Practical Diet With No Additional Mn On Physiological And Biochemical Indexes, Trace Elements' Contents Of Seminal Plasma And Testis Of Cockerels

The experiment was conducted to investigate the effects of three different treatments on manganese to physiological and biochemical indices, trace elements, contents in seminal plasma and testis of cockerels. 90 New Yangzhou cockerels of 83 weeks old was sampled, and randomly divided into three groupsⅠ,Ⅱ,Ⅲ, with 30 cockerels each. In the first two weeks, groupⅠand groupⅡwere supplied with practical diet with no additional manganese. After two weeks later, cockerels of groupⅠcontinued to intake the practical diet, nevertheless groupⅡwas survived with diet with 100mg/kg manganese (by means of MnSO4·H2O) until the end of the experiment. GroupⅢwas constantly supplied with diet with additional 100mg/kg manganese. The results showed that:1. At the beginning of the experiment, there was no significant difference of weights among each group. Then the weights of cockerels and testis index all presented upgrade tendency, there was no significant difference among three kings of treatments(P>0.05). It also showed that, because of the extension of illumination, the weights of testis raised.2. During the whole stage of the experiment, the succinic dehydrogenase activities (SDA) of each group presented upgrade tendency. There was no significant difference of SDA between groups.3.There was no significant differences of lactate dehydrogenase activities among each group(P>0.05). The stress of diets, alteration could raise the LDH activities of testis in short terms. Following the experiment, LDH activities tended to be steady after two weeks later, because of the comp. mechanism existed in cockerels.4. During the whole stage of the experiment, the density of testosterone increased. It was concerned with the seasonal breeding of poultry. The illumination stimulated the secretion of T through hypophyseoportal system. Adding manganese with short terms of manganese deficiency could increase the density of testosterone (T) in blood serum with significant differences in the fifth and the ninth weeks. Manganese could quickly be absorbed in blood, transferred in critical organ, stimulating the synthesis and secretion of T.5. It showed that adding manganese of 100 mg/kg in practical diet after two weeks of manganese exhausted restrained the absorption of manganese. The contents of ferric were discrepancy among individual.6. Be similar to mammalian animals, the content of zinc in seminal plasma of poultry was higher than other trace elements, it may be concerned with the important effects of zinc in animals, reproduction.7. The tendency of the contents of zinc in seminal plasma was similar to the contents of copper in seminal plasma, the density of testosterone in blood serum, and testis index. Id estⅡwas slightly higher than other groups. It showed that, zinc and copper in seminal plasma made profit to the reproductive performance of cockerels. Excessive ferric in seminal plasma may hold back the reproductive performances of cockerels.8. The androgone in seminiferons tubule of groupⅠaligned sparse, confused, and the order was not clear, Sertoli,s laid loosely. The order of the androgone in seminiferons tubule of groupⅡwas clear, compact, and the ripe spermatozoa in lumens were more.