Difference Of Pathogenicity On Poultry Embryo Fibroblasts Infected With Different Original Strains Of NDV

Newcastle disease virus (NDV)was found and isolated in 1926, and prevalence of the virus has widely spread in the world. Although the virulence of different strains showed so many difference, in the past waterfowls and other birds were thought resistant to this virus, however, there has been some new pathogenic strains isolated from waterfowl.Experimental study was carried out about difference of pathogenicity on poultly embryo fibroblast infected with different original strains of NDV. Two strains were chosed to infect chicken embryo fibroblast (CEF), goose embryo fibroblast (GEF) and duck embryo fibroblast(DEF), for detecting comparative indictors as follows: the development of cellular pathogenic effect(CPE) and the title of virus by . Hemagglutin assay.The results showed that the most significant CPE infected with F₄₈E₈ 24 hours PI, the phenomena of the virus on DFF and GEF were slightly less. However, the pathogenicity in the cells infected with ZJ1 strains showed more remarkable plagues on GEF than on CEF or DEF, it indicted that pathogenicity on the monolayer was relative with the characteristics of the virus itself.The totle RNA of two strains were extracted respectively and used as the template amplify fusion gene(F gene). Fusion gene fragments of two strains were amplified by RT-PCR and subcloned into PGEM-T easy vector, the sequencing of 80.1 percent in the nucleotide sequence and 90.7 percent in the amino acid sequence, the difference sites of F gene in ZJ1 strain might be helpful to promote the cell fusion.The results might be a remarkable guidance to study the mutual relationship of NDV and the receptors on the cells. the emphasis in the future is the relationship of NDV and the receptors on different cells.