| The biological characteristics, the effect of different spore storage method and cultivate conditions on the formation of gametophyte and sporophyte in Adiantum reniforme var. sinense was studied in this paper The purpose of the study was to establish a base for breeding. The main points were as followings:The biological characteristics, including phonological features, growth processes and life cycle were observed so as to build the guideline for choosing excellent individuals and the methodology for breeding seedlings.The spores of A. reniforme var. sinense was non-chlorophyllous spores and could remain viable in ambient storage for at least five years, but different storage time has a significant effect on formation time of both gametophytes and sporophytes, with that 7 days ambient storage resulting gametophyte formation in 29 days prolonged five year ambient storage resulting sporophyte formation in 38 days, whereas 7 days ambient storage resulting sporophyte formation in 96 days prolonged four month storage resulting sporophyte formation in 105 days.Spore germination of A. reniforme var. sinense with wet or dry fronds that have produced spores was determined after 40, 72 and 112 days of storage in ambient, at 4°C and -20°C respectively. Whether the fronds were wet or dry had no signicant effect on germination percentage. But the germination percentage was decresed fastest at -20°C and lowest in ambient.The culture condition [controlled temperature and light: (25±1)°C and 2000∽25001x for 12 hrs/day]in sand media resulted in a shortest sporophyte formation time, 87 days after sporaphyte was inoculated. The sand medium is more favorable than peat soil for sporophyte formation and high density of gametophytes enhanced the sporsphyte formation. The sporsphyte would grow much stranger if the gametophytes were transplanted proper. |
