The Cloning And Primary Function Analysis Of Cotton ARF

Cotton is an important global economic crop. The cotton fiber is the main product, which has become more and more important for its advantages. Compared to other fibers, it has better traping body heat,better keeping moisture and permeable. In view of these nature advantages, the world has paid more and more attention to the quality of the cotton fiber. As the development of molecular biology,the use of genetic engineering to improve the quality of the cotton fiber has a good prospect of application and extension. For example, through seperating the aim sequence from the cotton genome and conducting it into the cotton, we find the fiber of the transgenic cottons is influenced. Though there have been many successful examples of cotton fiber improvement using genetic engineering technology, the report of ARFs influence on cotton fiber is still not reported recently.The auxin plays an important role in the process of plant upgrowth. It is reported auxin can promote the elongation of cotton fiber cell three days or two days before blossoming, the fiber cell can get elonggation ability throungh IAA-stimulation. Adding to IAA before blooming previously increases the cotton fiber quantity. ARF is the key factor in auxin signal transduction pathway, which can receive the signals caused by auxin and activate or repress the expression of the backward genes.In this research,through electronic cloning technology and bioinformation knowledge, we have seperated a new ARF gene by RACE technology, named ARF3. The GenBank accession number is EF467605. We have also got the whole cDNA sequence of ARF10 by RT-PCR method. We connect the two cDNA sequences to plant expression vector pCAMBIA1301, named pCAMBIA1301-ARF3 and pCAMBIA1301-ARF10. By vacuum infiltration technology, the two plant expression vectors have been transmitted into Arabidoposis thaliana. We have got many transgenic plants. The PCR detection proves the sequnece has successfully recombined with the genome of Arabidoposis thaliana. We have also proved its expression by the method of RT-PCR.We will take note of the phenotype of the transgenic plants after we get homozygotes.