| Microsatellite are short, tandenly repeating, l-6bp units of DNA that occur throughout the genome of many organisms. Microsatellite analysis is a method that had been widely used in many genetics reseaches such as gene mapping, population diversity analysis, population evolutionary analysis, parentage testing and QTL detections. In the present study, the polymorphism of 21 microsatellite loci which evenly distributed in all porcine chromosomes (except No. 18 chromosome) were analyed with the purpose to verify the genetic diversity in 5 inbred lines of Bama mini-pig . The results showed that the average observed number of alleles, effective number of alleles , observed heterozygosity, expected heterozygosity, polymorphism information content of 21 microsatellite loci in combined population from 5 inbred generations (generation 3, 4,5, 6 and 7) of Bama mini-pig were relatively low, which were 2.3180, 1.7683, 0.2904, 0.3434 and 0.3048, respectively. A relative high homozygosity rate (0.7096) was observed. The average homozygosity rate was increased, while the observed heterozygosity, expected heterozygosity and polymorphism information content decreased as the increase of inbred generation. The results suggested that the inbred population of Bama mini-pig is a relative homogeneous population and the breeding program and measures used to develope the Bama mini-pig inbreed strain were effective. |
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