Myostatin, Insulin-like Growth Factorsâ… ,â…¡ And Myosin Heavy Chain 2a Expression In Biceps Brachii Muscle Of Sheep

Myostatin (or GDF-8) is a member of theTGF-βsuperfamily, which is the negative regulator of muscle growth. The inhibition of Myostatin expression can accelerate the myoblast proliferation and myofibril hypertrophy, which results in the skeletal muscle hyperplasia. The objective of the study was to quantify and compare the expression of four genes in biceps brachii muscle of sheep(myostatin, insulin-like growth factorsⅠ,insulin-like growth factors II, myosin heavy chain 2a). The biceps brachii muscles derived from the lambs (Dorper×Small-tail Han sheep) from 1 day to two month after birth were sampled. The relatively transcriptional abundances were measured using Realtime RT-PCR.Our results documented that the expression regulation of IGF1,IGF2 and MSTN genes was different in biceps brachii muscle. obviously of IGF1 was the lowest and the expression of MHC2a was the highest during four genes. Transcript level was not obviously different between MSTN and IGF2. The expression of MSTN reached the highest point at 7 day and droped to the lowest point at 7 weeks after birth. The expression of IGF1 arrived at the highest peak at brith, and degraded the half of the birth after 7 days. Then, the change of the IGF1 expression level was no abvious. The expression result of the IGF2 decreased to the least at 1 week and increased the max point at 3 weeks. The change regulation of IGF2 expression was showed as a wave. The expression level of MHC2a reached the highest point at birth and achieved the 1/7 level of the highest point.Correlation of MSTN and IGF1,IGF2,MHC2a was not significantly statistical, it showed that there were no exact control mechanisms between MSTN and other genes. Correlation of IGF1 and MHC2a was significantly statistical, it suggested that the interaction exited between them.