QTLs Analysis Of Quality Characters In Maize In Different Developing Stages

In this investigation, a set of incombant inbred line (RIL) population which derived from an elite hybrid maize Nongda108,were used to dissect the genetic basis of three main nutrient components in maize kernels under different developing stages, the RIL population was evaluated in three locations including Nanyang, Zhengzhou and Xunxian in Henan province. The results were as follows:1) A genetic linkage map with 123 marker loci was constructed including 10 chromosomes; the total length was 2076.1cM, with an average interval length of 16.88cM.2) Three nutrient components of kernel protein, starch and oil in seed were measured by using NIRS in four developing stages.3)A total of 58 QTL associated with nutrient components in maize kernels were detected using composite interval mapping method (CIM) in RIL population. There were 23 QTL associated with protein content, and the QTL lied on all chromosomes except 8. There were 20 QTLs associated with starch content, and the QTL located on all chromosomes except 7 and 8. For oil content, 15 QTL was identified, which lied on chromosomes1, 2, 4, 8, 9, and 10, respectively.4) Through conditional QTL analysis, 8 QTL was detected for protein contentd in four developing stages, and the QTL lied on chromosomes 1, 2, 4, 7and 9, respectively. There were 5 QTLs associated with starch content, which lied on chromosomes 1, 5 and 6. For oil conten, there were 3 QTL identified, which lied on chromosomes1and 6. The results suggested that the expression of the QTL controlling nutrient component was different in different developing period, which has significant interaction with environment.