Study On Gene Heredity And Expression Of The Ipt-bar-transgenic Rice

This experimentation researched the offspring populations that ipt-bar-transgenic rice hybridized with Shu-hui 725 and Shu-hui 527 in the heredity and expression. The results as flows:1. Differently the ipt-transgenic rice plants in backcross generation of community discovered respectively 15 individual plant; the proportions were approximately 50%, the performances were 1:1 separation. That was discovered respectively 61. 73% and 60. 27% to the different backcross two generation of communities, it displayed 1:1separation. Therefore the ipt-transgenic rice communities are a pair of dominant gene control in the backcross generation. The masculine rice plant was 135 in F₂ of ey105×Shu-hui725, about 72.2%. The masculine plant in F₂ of ey105×Shu-hui527 was 146, in 74.9%. Two F₂ communities conform to 3:1 separation rule, the performance are a pair of dominant gene control. Therefore the ipt gene conforms to the Mendel in the backcross generationand in the hybrid descent to separate the rule.2. The separation rule was differently in bar-transgenic BC₁F₂ and F₃hybrid descendants. Some conform to 3:1 separation; some conform to 2:1 and 1:1 separation. The main performance is 1:1 and 2:1 separation in BC₁F₂, but the performance is 1:1 and 3:1 separation in F₃.3. The cold tolerance performance of transgenic ipt plants in rice showed that the relative conductance of ipt-transgenic rice is smaller than the contrast. The destroyed chlorophyll of ipt-transgenic rice is reduced under the low-temperature. The root activity of ipt-transgenic rice is larger than the contrast. And it is greater than ipt transgenic rice that low-temperature impact on yield of contrast. Finally, taken the analytic approaches of grey related degree, it is analyzed and judged that every index has relation with the single output in different treatments. There are indexes including the rate of conductance, root activity, chlorophyll a/b and heavy of a hundred to exert a great influence on thesingle output under the low temperature.4. To bar-transgenic plants BC₁F₂ and F₃ of ey105×Shu-hui725 and ey105×Shu-hui527 and the contrast sprayed 250mg/L PPT. Results were the control plant died completely, but hybrid masculine plants can normal growth, because it included the herbicide-resistant gene.5. After ten days transplanting of bialaphos-sodium 250mg/L testing the results showed that the height of rice plants, ear length, and 1000-rates of greater impact reached a very significant difference (F=9.4807**, 9.986**, 21.5948*. 10.5074**); ear to achieve significantly higher levels (F=3. 6379*) ; BC₁F₂ reached significant difference with F₃ and all the bar-transgenic generations have very significant difference with contrast.6. Trial was carried out 5% glyphosate after transplanting ten days. Results showed that the contrast was all death. But the bar-transgenic plants grow normally. Different generations of bar-transgenic plants were greater impact by glyphosate 5% in the height, seed setting rate and 1000-weight. Plants height levels of different generations bar-generational differences were significant (T=2. 7917*).7. After analysis different herbicide treatments we can see that bar-transgenic BC₁F₂ achieve significant with F₃ after carrying out 5% of glyphosate. After adopted 250mg/L bialaphos-sodium different bar-transgenic generations were not significantly. But bar-transgenic generations got significant between contrasts. In the same generation (BC₁F₂), the different herbicide treatments achieved a significant level. But F₃ no have significant difference between the treatments.