The Affect On The Protein Metabolism In Animal By Exogenous CNT

Haerbin white rabbits were used as experimental animals in this research to study the dynamic disciplinarian of how 3H-Lysine was absorbed, distributed, transformed, metabolized and finally egested, and discussed protein biotransformation processes in vivo with isotopic tracer technique, radioimmunoassay and compartment analysis classical theory. Furthermore to research the variety of blood's main biochemical and physiological values, endogenous CNT content and related genes in the process of protein metabolism by injecting exogenous CNT. Moreover, it explored the regulation mechanism of exogenous CNT for animal protein metabolism, so as to construct the CNT dynamic metabolism model. And provide foundation for the control of the protein metabolism in big animals such as cattle and sheep. The results were as follows:1. The metabolism of 3H-Lysine in animal could be described with two compartment model of first–order process, and the optimum dynamic equation was gotten with fitting method, after improving the model. In the equation, there were some indexes as follows: The determination coefficient (R2) was 0.9989.After injecting 3H-Lysine, it entered into central ventricle and surrounding ventricle simultaneously, and in the rate of later one was 99.62%.It reached equation with the disappearance of central ventricle and the reversible transformation .And then transformed to other organs through blood ,reached equation after 30h, and at last disappeared through organs.2. The result of testing organic samples showed that in normal physiological condition, the majority of 3H-Lysine participated the protein synthesis of kidney, heart, muscles, spleen and other organs besides a small part was discharged out.There were obvious changes of content of 3H-Lysine in many tissues or organs after injecting CNT(p<0.05). In cAMP group, it deposited mainly in muscles , stomach, liver, heart, and procreant organs; in cGMP group, it deposited mainly in bladder, muscles, lung, intestines, and fat organs; in cGMP+cAMP group, it deposited mainly in muscles, bladder, procreant organs, fat organs and stomach. 3. By testing, the endogenous cGMP and cAMP concentrations of blood were separately 22.72 pmol/mL and 88.84 pmol/mL, and cAMP/cGMP was 3.91 in rabbits. Moreover, endogenous CNT could be improved availability by injecting exogenous CNT. So injecting cGMP alone could increase endogenous cGMP and cAMP 7.40pmol/mL and 17.20pmol/mL separately, and the increasing rate were 32.54%and 19.36%. And injecting cAMP alone could increase endogenous cGMP and cAMP 3.05pmol/ml and 34.50pmol/ml separately, and the increasing rate were 13.41%and 38.84%. And injecting the admixture of cGMP and cAMP with the proportion of 1:1 could increase endogenous cGMP and cAMP 4.42pmol/mL and 22.79pmol/mL separately, and the increasing rate were 19.42%and 25.66%. In addition, injecting cGMP alone could increase significantly endogenous cGMP, and there was the best effect in 45h. So did injecting cAMP, but the effect was durative in 4d or later.4. The results of test showed that the concentration of blood growth hormones were all higher than control group (13.89ng/ml), the average concentration of cGMP group, cAMP group, and mixed group were: 19.84 ng/mL,24.38 ng/mL and 20.34 ng/mL,the average amplitude were: 42.90%,75.61% and 46.50%, all of them had significant change(p<0.05)and great significant change (p<0.01)compared with control group, the increasing range of content of GH about these three were: from28.41% to 64.27%,from 25.71% to 114.00% and from 29.90% to 73.93%. There was different variation of content of insulin compared with control group, the average concentration of cGMP group, cAMP group, and mixed group were: 706.30μg/ml,495.67μg/ml and 636.42μg/ml, the average changing rates were: 20.43%,-15.48% and 8.51%.The results indicated that the level of insulin in blood was regulated positively (ascend) by cAMP and negatively (descend) by cGMP. The content of testosterone had a different variation compared with control group(146.96ng/ml), the average concentration of cAMP group,cGMP group and mixed group were: 433.81 ng/ml,24.38ng/ml and 234.91ng/ml, the average changing rates were: 195.18%,-83.41% and 59.85%. All of them reached significant chang(ep<0.05)and great significant chang(ep<0.01)compared with control group, the increasing range of contents of testosterone about these three ones were: from 43.23% to 330.22%,from -79.62% to -87.41%% and from 23.13% to 154.12%.5. In normal physiological condition, the average content of blood fat,blood sugar and blood protein in rabbits were 1072mg/100mL, 80.11mg/100mL, and 8.37g/100mL separately. After injecting cGMP alone, the blood fat content increased rapidly, about 17.63% averagely, but the blood fat content after injecting cAMP decreased slightly. After injecting cGMP alone, the blood sugar content increased above 10% averagely, but the blood sugar content after injecting cAMP increased only 1.79% averagely. After injecting CNT, the blood protein content increased about 5% averagely. The change range and frequency of the blood protein content was approximately consistent with .the blood sugar.