Extraction And Analysis Of Active Components In Bark Of Juglans Mandshurica

The active components in bark ofJuglans mandshurica are extracted by 95% alcohol,and then the extractives are separated by petroleum ether, chloroform and ethyl acetae inturn. The results of activities test of all these extractives showed they all had insecticidalactivities to Lymantria dispar and they can all make Lymantria dispar refuse eating food.The chloroform and ethyl acetae extracts have stronger antifungal activities, The MIC ofthem is 3.125 mg/ml to Alternaria alternata. Activities tests not only point out way foranalysis and extraction of active compound in bark ofJuglans mandshurica, but also givescience information for the application.Chemical methods and GC-MS methods are used in turn for analyzing the chemicalcomponents of chloroform and ethyl acetae extracts. The results of chemical tests showedthat there are hydroxybenzenes and tannin, naphthoquinones, flavones, lactones,cumarines in the ethyl acetae extracts, there are probably organic acid,but notanthraquinones and its glucide in the ethyl acetae extracts, the analysis of GC-MS affirm14 compounds of them, 94.61% of ethyl acetae extracts, the most compounds are 1,2,3-Benzenetriol, 5-hydroxy-1,4-Naphthalenedione and 7-Methoxy-1-tetralone,their,contentsinethyl acetae extracts are 42.31%,12.33%,11.26%. The analysis of GC-MS affirm13 compounds in chloroform extracts, the content in chloroform extracts is 94.04%, themost compound is 5-hydroxy-1,4-Naphthalenedione, the content in chloroform extracts is36.65%; TLC separation-HPLC test showed that the best spread solvents are chloroform:acetone=2:1 for chloroform extracts, and chloroform:acetone=8:1 for ethyl acetaeextracts. The analysis of chemical components may be consults for the separation andpurification of activities components.Extraction, stability, bioactivity and determination of weight content ofjuglone werestudied. The results show that the extraction yields are 0.001 31%, 0.001 26% and 0.00111% for ultrasonic extraction, conventional extraction and soxhlet extraction, respectively.High-purity samples of juglone were obtained by ultrasonic-aided extraction and vacuumdistillation, and the relative weight content was 96.420% according to the analyticalresults of GC-MS. The structural characteristics of juglone were also confirmed by colorreaction, alkali reaction, FT-IR and ¹H NMR, and a simple procedure of extraction wasdeveloped. It can also be found that, with strong anti-oxidation properties and weak anti-induction properties, the incorporation of Tween 80 was not corresponding with theincrease in decreasing rate, and pH and temperature had a striking effect on stability of juglone solution. Optimum stability was observed with pH 4, and the elevated temperaturehad a negative effect on stability. The experimental results also show that the extractedjuglone has good insecticidal activity and anti-fungi property, and effective prevention ofplant diseases, and the minimum concentration and pesticide time for prevention ofpoplar-leaves related diseases are 0.0625 g/L and 2d, and LC₅₀ for Lymantria dispar is0.83g/L. Spectrophotometric method was applied for the determination of juglone contentat wavelength of 426 nm, and the content and recovery rate were tbund to be 0.0933%and 99.6%, which further confirmed the data obtained from HPLC analysis.