Research On Genetic Transformation Of Two Different Stress Resistance Relative Genes To Improve The Abiotic Stress Tolerance Of Broussonetia Papyrifera L.

Broussonetia papyrifera L. Vent is famous for its application as raw material in paper industry and its availability in environment protection. Recently, it is regarded as to be a kind of potential oil plant for its high content of fibre. Given to our country's current problems that huge destruction of environment,the serious shortage of raw material in paper industry and insufficient fossil oil, it is high time for us to develop Broussonetia papyrifera(L.)Vent as a new kind of biology resources to meet the requirement of our country. Thus, how to improve the stress tolerance of Broussonetia papyrifera(L.)Vent so that it can be planted in more places in our country has increasingly been focused on by researchers.Through the Agrobacterium-mediated transformation, two different stress-tolerance related genes, otsA and AtNHX5 are transferred into the Broussonetia papyrifera(L.)Vent. After selection, regeneration and PCR assay, 5 otsA transformant were obtained and 8 AtNHX5 transformant were ontained.After comparing the tolerance of the control plants and the transformant in the stress of low temperature,high salt and drought, we figured out three otsA transformant, otsA3,otsA13 and otsA18 with heightened drought tolerance and three AtNHX5 transformant, AtNHX5-5,AtNHX5-6 and AtNHX5-15 with heightened salt tolerance.Finally, through the RT-PCR to analyze the expression of foreign genes in transformants, the result shows that: The expression of otsA in Broussonetia papyrifera(L.)Vent might positively connection with its stress tolerance and there might exist modification after transcription about the AtNHX5 in transformant Broussonetia papyrifera(L.)Vent.