| In this paper,41 female New Zealand Rabbits were superovulated with FSH + hCG by muscle injected. Compared the influence about different FSH dose, season and the methods of constant and degressive on the results of superovulated. Analyzed the effects of different capacitation liquid, medium, the quantity of L-glutamine and the age of oocytes on the in vitro fertilization and zygote development. And studied the effects of the oocytes age on microinsemination, compared the results of microinsemination and normal in vitro fertilization. The results were as follows.1. Rabbits were superovulated with different dose of FSH, the results showed that, the average number of ovulation point and the average number of oocytes recovered from experiment group of 60 unit (36.13±8.13 and 28.88±6.71, respectively) and 100 unit (39.00±9.17 and 30.67±7.71, respectively) were more than which recovered from 40 unit (25.83±4.07 and 19.50±4.42, respectively, P<0.05). The average recovered rate (75.48%, 79.93% and 78.63%, respectively) between each other showed no significant difference (P>0.05).2. The average number of ovulation point (39.00±9.17), the average number of oocytes (30.67±7.71) and the average recovered rate (78.63%) recovered from spring were higher than that from autumn (21.58±7.10, 15.17±4.97 and 70.27%, respectively, P<0.05).3. Injected the same dose of FSH with the methods of constant and degressive, there was no significant difference between two groups (P>0.05). 4. The percentage of 8-16 cells of DM-His method (13.3%) was higher than that of DM and His methods (3.4% and 3.3%, respectively, P<0.05). The cleavage rate of the methods of DM, His and DM-His were 51.7%, 38.3% and 41.3%, respectively, there was no significant difference between each other (P>0.05), but the effect of DM method was a little better.5. The cleavage rate and the percentage of 8-16 cells from the age of 14-15h oocytes (41.3% and 13.3%, respectively) were higher than that of 16-17h oocytes (25.0% and 2.9%, respectively, P<0.05).6. Added L-glutamine in TCM-199 + 10% FBS could promote embryonic development, but as the quantity increased, the function was downing. The best adding quantity was 0.5 mmol/L.7. The cleavage rate, the percentage of morulae and blastulae from DMEM + 10% FBS medium (55.4%, 21.7% and 6.5%, respectively) were higher than TCM-199 + 10% FBS (41.3%, 6.7% and 0, respectively) and RM-199 medium (37.9%, 5.7% and 0,respectively, P<0.05); the percentage of 8-16 cells from RM-199 medium (25.3%) was higher than TCM-199 + 10% FBS medium (13.3%, P<0.05).8. After microinsemination with sperms, the morphologically normal rates of 19-20h oocytes (30.4%) was lower than that of 13-14h and 16-17h oocytes (61.9% and 64.6%, respectively, P<0.05), The cleavage rate from the age of 19-20h oocytes (42.9%)was higher than that of 13-14h oocytes (19.2%, P<0.05).9. The cleavage rate of normal IVF (41.3%) was higher than that of ICSI (22.9%) and Sham-operation (3.8%, P<0.05). It showed a significant difference between the cleavage rate of ICSI and Sham-operation (P<0.05).
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