Study On The Effects Of Two Derivates Of Glycine Replacing Antibiotics In Diet On Production Performance Of Broiler

The in vitro antibacterial activity of salicylaldehyde-glycine ligands and its metal complex with Cu(II) was studied and acute toxicological test on the complex with Cu(II) was conducted.Finally, the effects of the two derivates of glycine replacing antibiotics in diet on production performance, carcass characteristics and some immunity index of broiler was studied to evaluate the possibility of the two derivates of glycine replacing antibiotics in diet of broiler.The salicylaldehyde- glycine and its complex with Cu(II),Zn(II) and Co(II) were synthesized. The antibacterial activity of those ligands and complexes against three bacteria (E.coli, D.aureds, Salmonella) was determined by using concentration dilution method. The results showed that antibacterial activity of the salicylaldehyde-glycine was stronger against E.coli,Salmonella.The antibacterial activity of complex with Cu(II) was stronger against D.aureds.Among those compounds, the Cu(Sal-Gly)(H₂O) exhibited stronger antibacterial activity against bacteria in jejunum of broiler chicken.Eighty mice of Kunming breed, 1-day-old, about 14g of live weight, half of female and male, were used in this experiment. When the mice were 18～20g of live weight, they were divided into control, test groups of I, II and III (with 20 mice in each group). The animals were poured 80% of dimethylsulfoxide through mouth and the mice in test groups of I, II and III were poured 1500mg/kg, 1000mg/kg, 500mg/kg Cu(Sal-Gly)(H₂O), respectively. The result showed that the LD50 >1000mg of Cu(Sal-Gly)(H₂O)/kg.Sixty 1-day-old AA+broiler chickens were randomly divided into control group and test groups of I, II, III and IV(with 12 broliers were in each group). The control group was fed diet supplemented with antibiotics. Birds in the test group I and II were fed diet replacing antibiotics with 0.55g and 1.1 g of Salicylaldehyde- glycine /kg diet respectively. Chikens in the test group III and IV were fed diet replacing antibiotics with 0.37g and 0.74 g of Cu(Sal-Gly)(H₂O) /kg diet respectively. The results showed that the two derivates of glycine improved the growth performance, brisket rate, leg portion rate, the indexes of immune organs;decreased abdomen fat of broilers on the 42nd day to some degrees. Average daily gain of birds in group I, II, III, IV was increased by 8.53%(P<0.05),2.57%,7.00%(P<0.05) and 1.75% respectively.Brisket rate was improved by 12.93%(P<0.05),4.23%,4.80% and 2.79% respectively. Leg portion rate was improved by 13.09% (P<0.05), 10.79% (P<0.05), 11.34% (P<0.05) and 6.94%, respectively. Splee comparative weight was increased by 54.92% (P<0.05), 26.49%, 34.44% and 2.03%, respectively. Fabricius comparative weight was increased by 83.3% (P<0.05), 36.90%, 71.43% (P<0.05) and 21.43%, respectively. Abdomen fat was decreased by 18.69%, 17.76%, 33.64% (P<0.05) and 42.52% (P<0.05), respectively. It showed that the effect of the low levels of the two derivates of glycine on production performance of broiler was better than that of high level.In conclusion, the two derivates of glycine have property of inhibiting bacteria. Among those compounds, the Cu(Sal-Gly)(H₂O) exhibited stronger antibacterial activity against bacteria in jejunum of broiler chicken. The LD50 >1000mg of Cu(Sal-Gly)(H₂O)/kg. Considering the effect of the two derivates of glycine on growth performance,carcass characteristics and some immunity indexes on broilers, replacing antibiotics with the two derivates of glycine in broiler diet is practicely and supplementation with the levels of 0.55g of salicylaldehyde-glycine ligand 0.37g of Cu(Sal-Gly)(H₂O) in per kg of diet had the best effect on broiler.