Study On Photoautotrophic Micropropagation Technique Of Acacia Crassicarpa And Acacia Melanoxylon

In this experiment ,we studied the Photoautotrophic micropropagation of Acacia rassicarpa and Acacia melanoxylon in the phase of rooting and acclimation using Photoautotrophic micropropagation system designed by ourselves .And then compared with traditional tissue culture,we proved the feasibility of our study. The combination of traditional tissue culture technology and Photoautotrophic micropropagation technology made tissue culture technology consummate and develope farther,and inaugurated a new approach for the rapid propagation technology of Acacia, plantlets.It will be the rapid propagation technology that is adapt to the production of plantlets in south part of china and have its own intellectual property rights.The main results were described as following:1. In the process of Photoautotrophic micropropagation ,the effect of rooting was different in distinct porous culture medium and nutrient solution about two different Acacia.Both Acacia crassicarpa and Acacia melanoxylon obtained better rooting effect in sand culture medium ,and their rooting effects were well and their rate of rooting was highest in water culture medium.The rate of rooting of Acacia crassicarpa reached 92% and the rate of rooting of Acacia melanoxylon reached 89%.2. In the process of Photoautotrophic micropropagation .different treatments of antimicrobial tuzet in the agar culture medium had different effect's on the rate of rooting and the rate of contamination of two different Acacia..NO.7 (tuzet,500 mg/kg,5mL)was the best treatment of antimicrobial in the surface of culture medium,and the rate of rooting of Acacia crassicarpa was 83% and the rate of contamination of Acacia crassicarpa was 10.07%,that of Acacia melanoxylon were 86% and 9.02%.3. The effect of rooting of two different Acacia, was distinct in different light intensity pattera,and both of them developed better in NO.2 light intensity pattera(in 0-7 d, light intensity was lower.controlled between 2500-3000 lx,the hours of light were 12 h/d,in 8-10 d light intensity reached 4000-5000 lx, the hours of light were 14 h/d,after 10 ds light intensity reached 6000-8000 lx, the hours of light were 14 h/d). In this pattern(other environment factors were unchangeable),every index (the rate of radication,the biomess of plant over soil and under soil) in agar culture medium and sand culture medium was better than other patterns,and the rate of rooting of Acacia crassicarpa in each culture medium was 84% and 92%,the biomess of plantlet over soil was 27.6 mg and 25.6 mg ,the biomess of plantlet under soil was 4.1 mg and 5.2 mg. Acacia melanoxylon was similar to Acacia crassicarpa. The rate of rooting of Acacia melanoxylon in agar culture medium and sand culture medium was 85% and 90%,the biomess of plantlet over soil was 20.7 mg and 19.8 mg,the biomess of plantlet under soil was 3.5 mg and 4.0 mg.4. In the contrast investigation of Photoautotrophic micropropagation ang traditional tissue culture about rooting culture of two different Acacia., our research found that the rooting effect of Acacia crassicarpa was the best in sand culture medium of Photoautotrophic micropropagation .The rate of contamination(0),the root vigour (2997.501 mg/(g ? h)),the biomess of plantlet under soil(5.3 mg),the survival rate of transplantation(90%) were better than that of Acacia crassicarpa rootage plantlets in the two other culture methods besides the middle level of rooting ratio and chlorophyll content and biomess of plantlet over soil .In addition, the rooting rate of Acacia crassicarpa was highest as 95% in traditional tissue culture ,the chlorophyll content and biomess of plantlet over soil of Acacia crassicarpa was highest as 1.47 mg/g and 27.3 mg in agar culture medium of Photoautotrophic micropropagation mode. The rooting effect of Acacia melanoxylon was the best in sand culture medium of Photoautotrophic micropropagation too. Besides the middle level of rooting ratio,chlorophyll content and biomess of plantlet over soil.the rate of contamination(0). the root vigour (2374.781 mg/(g ? h)),the biomess of plant under soil(4.2 mg),the survival rate of transplantation (93%) were better than that of Acacia melanoxylon rootage plantlets in other culture methods. In addition,the rooting rate of Acacia melanoxylon was highest as 94% in traditional tissue culture,the chlorophyll content and biomess of plant over soil in agar culture medium of Photoautotrophic micropropagation mode was highest as 2.30 mg/g and 21.0 mg.5. Compared with traditional tissue culture technology,the Photoautotrophic micropropagation technology could reduce production cost notably as well as improve production rate,and the production cost of Acacia crassicarpa reduced about 45% and that number of Acacia melanoxylon was 43%.