| The polymorphisms of five genes (PIT-1, MyoG, UCP3, H-FABP, RN) were genotyped by PCR-RFLP in the populations of Chalu, PC (Pietrain×Chalu), Jiaxing, Jinhua (Line I) pigs (about 283 pigs) in this experiment, and then SPSS software was applied to analyze the relationships between PIT-1, MyoG, UCP3 and H-FABP genes and the growth traits (including birth weight, 21d, 35d, and 60d-body weight), carcass traits (including head weight, body weight, ham weight, ham muscle weight, ham fat weight, lean meat percentage, body length, backfat thickness, longissimus dorsi area, leaf fat weight, and hoof weight) and meat quality traits (including meat color, muscle pH, muscle temperature, muscle electrical conductivity and water holding capacity), and the results are the following.There were three genotypes (AA, BB and AB) of PIT-1 gene in Chalu pig, while two genotypes (BB and AB) in PC pig and only one genotype (AA) in Jiaxing pig. Genotype AA of MyoG gene was the most popular one in Chalu pig (84%), while BB was the least one (only 4%), and the same distribution was found in PC pig. The frequency of allele A was 90% in Chalu pig and 75% in PC pig, while the frequency of allele B was about double times as that of A. Only one genotype of this gene was found in Jiaxing pig. There were three genotypes of UCP3 gene in all of the three populations (Chalu, PC and Jiaxing), the frequency of AA was nearly equal to that of BB, which was also found in Jiaxing too, genotype AB was the most popular one (49%) in Chalu pig, and the most popular one was BB (60%) in PC, genotype AA was the least one in Jiaxing pig. There were three genotypes of H-FABP (hh, Hh and HH) in Chalu pig, while there was no heterozygote in PC pig, and there were contrary distributions of allele H and h in the two populations. The frequency of RN~- of which was lower than that in Hampshire while higher than that in Landrace and Yorkshire pig was 0.3657, which was just adverse in Jinhua pig.PIT-1, MyoG, UCP3 and H-FABP genes had no significant effect on the growth traits in Chalu pig. PIT-1 gene had a significant effect on body length, but no significant effect on other carcass traits and meat quality traits. The two homozygotes of MyoG gene (AA and BB) had significant effects on head weight, body weight, ham weight, ham muscle weight, ham fat weight, body length and hoof weight (P<0.05), but no significant effect were found on meat quality traits. The different homozygotes of UCP3 gene (AA and BB) had significant effects on body weight and average backfat thickness. Significances were found between genotype AA and BB of this gene and body weight, shoulder fat thickness and buttock fat thickness, while AA and AB type had significant effects on shoulder fat thickness, thorax-waist fat thickness, average backfat thickness and leaf weight (P<0.05). The genotype AB and BB of UCP3 gene had significant effects on L value, b value, muscle pH for biceps femoris (BF), longissimus dorsi muscle temperature and electrical conductivity, while genotype AA and BB also had a significant effect on longissimus dorsi muscle temperature (P<0.05). The two homozygotes of H-FABP gene (hh and HH) had significant effects on head weight, body weight, ham weight, ham muscle weight and body length. Significance were found between Hh and HH and ham muscle weight (P<0.05). The effects of genotype Hh and hh on a and b value were nearly significant (P was respectively equal 0.054 and 0.062), genotype Hh and HH had a significant effect on water holding capacity (P=0.040<0.05). There were no interactions among the four genes on body length, body weight, head weight, ham weight and ham muscle weight in our experiment. |
PDF Full Text Request