Study On Molecular Ecology Of Microbe Related With Cellulose Decomposition In Staw-Amended Soil

Straw are a crucial source of nutrients in both natural and agricultural ecosystems. It is a very essential method for soil fertility, quality and healthy by amending straw to the soil. The microorganisms in soil play an important role during the process of the straw decomposition after the straw was amended to the soil. The object of our study was an investigation on microorganisms related with cellulose decomposition in straw-amended soil. There are three parts in this study.In the first part, the diversity of soil microbe cultured at different cellulose enrichment culturing conditions was analyzed by molecular technology of DGGE combined with statistical methods of clustering analysis and PCA. Both cluster dendrogram analysis and PCA analysis demonstrated that different cellulose enrichment culturing conditions might cause different effect to the soil microbe. The structure and components of the samples incubated in CMC medium and PCS medium at 50℃showed a relatively high level of similarity, In contrast, those incubated in CMCmedium and J medium were significantly different...