The Detection Of The Contents Of ATB1 And AFTM1 In Livers, Kidneys And Tissues Of Pigs Fed Wish Restaurant Wastes

In recent years, wastes from restaurants have been used to feed pigs in outskirt areas of some cities in China. Therefore, a problem on food safety rose with the events. For toxin always produced in the bad storage and transport processes when the wastes began to rot. After feeding to pigs, toxin would accumulate in splanchnic organs and muscles in those pigs. And then the toxin containing in pig bodies would enter into the food chain of human beings, which was a potential hazard to human health. So it is necessary to assess the toxin contents in those pigs, to find out whether is it always over than the limited levels of the state standards?To test the toxin contents in pigs, the experiment was designed and implemented as follows: 20 pigs randomly selected from 4 different hogpens using restaurant wastes as feed were treated as experimental group, and 5 pigs from a normal feeding hogpen were treated as controls. Indirect competitive enzyme-linked immumosorbent assay and immunoaffinitycolumn-fluorospectro -photometry method were used to test the aflatoxin B₁ (AFTB₁) and M₁ (AFTM₁) contents in livers, kidneys and muscles in all pigs. The results shown that two type toxins were detected in all organs and tissues in all pigs in experimental group, which reached to 3.682μg/kg of AFTB₁ and 0.158μg/kg AFTM₁ in average. The AFTB₁ contents were higher than AFTM₁ in same tissues or organs. Otherwise from AFTM₁, which were almost stable in all organs and tissues in one experimental pig, AFTB₁ contents varied in different organs and tissues, the liver and kidney contents were significantly higher than muscle contents and the liver levels were the highest. But in all controls, any of two type toxins were all undetectable.Although there were few cases that aflatoxins contents in tissues and organs in experimental pigs were higher than the national limited standards(AFTB₁ 5μg/kg and AFTM₁ 0.5μg/kg), but the aflatoxins contents in some pigs were approaching this level. The above results indicated that feeding pigs with restaurant wastes was an incorrect action in pig industry, which may pose an additional threat to human health, so the ways for pig feeding should be forbidden.