Effects Of Microbial Ecological Agents And Compound Enzyme Preparation On Performance And Digestive Physiology Of Allogynogenetic Crucian Carp

The purpose of this experiment is to investigate additives' influence on performance, the activities of digestive enzyme, intertinal microorganisms and trypsin mRNA expression in hepatopancreas of Allogygonetic Crucian Carp. A case study of Allogygonetic Crucian Carp was performed in this project by adding different additives (e.g. Bacillus.licheniformis, Xylooligosaccharides and compound enzymes preparation) in the diet. The application of the study is to provide more theoretical evidence for the research of these additives and their compatibilities in aquatic nutrition science. It also gives scientific instruction to aquaculture.EXPERIMENTIn the experiment, 200 Allogygenetic Crucian Carp(average initial weight, 30±2g) were sorted into four groups randomly and named as control Ⅰ, experiment Ⅱ, Ⅲ and Ⅳ. Fish in different groups were raised for 100 days by feeding with different food. Control I feed with basal diet, 0.01% Bacillus licheniformis was added to diet for group Ⅱ, while 0.01% Bacillus licheniformis and 0.01% xylooligosaccharides were added into food for group Ⅲ, and diet for group Ⅳ were mixed with 0.01% Bacillus licheniformis and 0.03% compound enzymes preparation. The following indicators of fish were measured and analyzed for each group: performance, apparent digestibility coefficient of proteins and dry matters, protease activities of intestine and chime, the amount change of intestinal microorganismsThe results of the experiment indicated that the apparent digestibility coefficient of proteins of fish in group Ⅱ was 7.36% higher than that in control, while the results from both group Ⅲ and groups Ⅳ were 7.07% higher and were significantly difference(P<0.05). The apparent digestibility coefficients of dry matters in all experiment groups were improved obviously: the result of group Ⅱ was 74.84% higher comparing to the control,...