Preliminary Studies On In Vitro Culture And Secondary Metabolites Of Aloe

Aloe, with high value of medicine, cosmetics and health care, is liliaceae.Aloe has produced some important secondary metabolite materials, such as anthraquinone. These experiments have researched on the aspect of tissue culture system; induce callus, suspension culture and component mensuration of A.saponaria and A.arbescens Mill. As the results, A.saponaria can produce active substance components by secondary metabolite culture and lay on a theoretical basis to improve the further development of aloe in the industry.In these experiments dropped the aloe explants into the liquid containing the sodium hypochlorite 2% for 12min .6-BA is the most important factor in rapid propagation system. Rooting is stimulated more effectively with IBA.Inducing callus and rapid propagation system research has proved that the best part of root. The best inducing callus medium of A.saponaria is MS+6-BA 3.0mg/L +2,4-D 3.0mg/L+NAA 2.0mg/L+LH 100mg/L+AA 100mg/L and callus weighted 5.166g on the medium added AgNO315mg/L.In suspension culture, the cells in dark grow fast in the medium of B5+2,4-D 3.0mg/L+KT 0.1mg/L+sugar20g/L. The growth trend of calli and suspension cells are recorded in a logistic growth curves (S type), respectively, and the calli maximum growth is the best product tach 25days.Secondary metabolite culture research has showed anthraquinone production high yielded with yeast extract and Cu+ of the S type curves.To solve browning problem, we based on the principles of the phase of inducing callus and the phase of suspension culture. Results show that the antioxidizing agent is better than the adsorbent. In suspension culture phase found the effect on the browning by added the antixidizing agent and the adsorbent.