Influence Of Cysteamine On Antioxidant Capacity, Hemoglobin Content And Utilization Of Iron

Iron is one of the most important trace elements. Different iron sources may influence iron absorption and antioxidant capacity. Less is known about the antioxidants could enhance utilization of iron. In this thesis, different doses of cysteamine(0,40,80mg/kg)as antioxidant were supplemented to the chickens and anemia rats'diets which contain different iron sources (ferrous sulphate and methionine chelated iron). Studies conducted the effect of cysteamine on antioxidant capacity, hemoglobin content and utilization of iron.Effect of cysteamine on antioxidant capacity was firstly studied. Compared with control group(0mg/kg), the glutathione (GSH), total antioxidate capacity (T-AOC) and glutathione peroxidase (GSH-Px) in plasma of chickens and anemia rats in the groups of supplying with 80mg/kg cysteamine were significantly increased (P<0.05), and the concentration of maleic dialdehyde (MDA) was decreased (P<0.05). Whereas the activity of catalase (CAT) in plasma of anemia rats was not significantly (P>0.05) increased. There were significant differences in GSH content and GSH-Px between two iron sources in chickens, so was GSH-Px in anemia rats.It was aimed to examine the influence of antioxidant capacity on hematogenic function by measuring concentrations of hemoglobin. Concentrations of hemoglobin of chickens were significantly increased after supplying with cysteamine (P<0.05), especially in 80mg/kg dose. When anemia rats were fed with cysteamine for 1 day, concentrations of hemoglobin were improved rapidly. Compare with control group, concentrations of hemoglobin in ferrous sulphate groups supplemented with 40 and 80mg/k cysteamine were increased 10.34% and 19.44% respectively, and 13.42% and 31.16% were increased respectively in those of methionine chelated iron grouops. As the doses of cysteamine were the same, methionine chelated iron was better than ferrous sulphate. Correlation of T-AOC and hemoglobin showed that advance of antioxidant capacity enhanced concentrations of hemoglobin, and promoted the body hematogenic function.Finally the effect of cysteamine on utilization of iron was evaluated. With regard to chickens, both plasma and hepatic iron levels were increased (P<0.05), while there was no impact on total iron-binding capacity (TIBC). As to anemia rats, hepatic iron level was significantly higher than control group (P<0.05), so was plasma iron level after being iron fortified for 1 day and TIBC after being iron fortified for 4 days (P<0.05). Efficacy of methionine chelated iron for improving iron nutritional state was superior to ferrous sulphate. Correlation of T-AOC and plasma iron, hepatic iron levels revelaed that increase of antioxidant capacity enhanced the utilization of iron.In conclusion, the antioxidant capacity was improved by adding cysteamine to diets.