| This study was conducted to evaluate the effects of different organic iron sources on growth, haematological and immunological characteristics of piglets, and study the absorption and utilizing of different organic iron for choosing optimal iron source. This study was designed in two phases, iron status was adjusted in first phase. Fifty27-day-old piglets (LandracexYorkshire) weighing7.27±0.95kg were choosed, fourty-five piglets were feed iron deficient diet to establish iron deficiency model, five piglets as control were feed iron deficient diet+100mg/kg FeSO4.In the second period, all pigs of iron deficient diet of the first period were allotted to another five dietary treatments based on body weight,hemoglobin concentration(HGB) for28day feed trial. All dietary treatments were feed basal diet and120mg/kg iron as FeSO4, Fe-Gly, Fe-Lys, Fe-Met and ferrous fumarate, respectively. Blood sample was collected every week in the first period and on d0, d14, d28in the second period. Four piglets of each treatment were slaughtered for collecting heart, liver, spleen, lung, kidney tissue and duodenum mucous. Spleen, mesenteric and inguinal lymph nodes, and thymus land were separated and weighed.Blood routine indicators of HCT, HGB, RBC, RDWcv correlated with iron status of piglets were determined every week in the first period. Growth performance, blood routine indicators, serum iron and TIBC, ferrtin, transferrin and immune organs index, minerals content in tissue, digesta pH of duodenum, were determined throughout the second period of our study. HAMP, DMT1, FPN, IRP that related with iron absorption gene were determined.The results1) Piglets of iron deficient diet decreased in HCT (p<0.05), HGB (p<0.05), RBC (p>0.05), RDWcv (p>0.05) on day21compared with day0. Time had no effects on these indicators of control.2) On the first week of the second period, all organic iron increased ADG (p>0.05); Compared with Fe-Lys, Fe-Gly increased ADG(p<0.05), compared with Fe-Lys, Fe-Met, FeSO4Fe-Gly decreased F/G on the second week(p<0.05).3) Fe-Gly had the trend of increasing HCT, HGB, RBC. On14d, Fe-Gly increased serum iron(p>0.05), iron saturation level (p>0.05), ferritin(p> 0.05),decreased transferrin (p<0.05);all organic iron treatments had decreased total iron binding capacity(T1BC), increased iron saturation level(p>0.05). Same changing trend existed on d28.4) Fe-Gly increased serum CAT activity significantly (p<0.05),decreased MDA concentration (p>0.05),other organic iron had the trend of increasing CAT activity(p>0.05).Organic iron treatments had no significant effects on SOD and MDA.5) Organic iron treatments had the trend of increasing mesentery lymph gland, total lymph gland index(p>0.05). Organic iron treatments increased serum IgG, IgM, IgA, and Fe-Gly had higher IgM, IgA than control (p<0.05).6) Fe-Gly increased Fe content of heart, liver, spleen, pancreas, muscle,25.6%(p>0.05),9.1%(p>0.05),2.4%(p>0.05),48.6%(p>0.05),3.1%(p>0.05),34.4%(p>0.05), respectively.All treatments had no significant effects on Cu,Zn content of tissue (p>0.05). Fe-Gly decreased Fe of feces (p<0.05), Fe-Gly had lower value than control (p<0.05).7) Fe-Gly decreased the expression of HAMP significantly (p<0.05), and increased the expression of FPN. Fe-Lys had decreased the expression of IRP2(p<0.05).The conclusions:1) Addition of120mg/kg iron of Fe-Gly improved growth of piglets, increased ADG and coat index.2) Organic iron had better effects on improving immunity.3)Fe-Gly increased iron absorption by decreasing the expression of HAMP, increasing FPN, DMT1. |
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