Pharmacokinetics And Residue Depletion Of Avilamycin In Carp

Avilamycin is an oligosaccharide mixture, composed predominantly of the two compounds A and B produced by Streptomyces viridochomogenes, belongs to the orthosomycin group of antibiotics. Avilamycin is approved for use as a growth promoter within the European Union in the swine and poultry industries but not for therapeutic use. Nowadays, the consumption of aquatic product is increasing fast, especially in developing countries. The augment of drug in aquaculture exploits to promote and cure the fisheries from disease. In this research, we established method to determine avilamycin in carp plasma and tissues. Pharmacokinetics and residue depletion of avilamycin were studied in carps. The work is to reveal the absorption, excretion and depletion of avilamycin in carp, further to determine the marker residue and target tissue, and to estimate a withdrawal time of avilamycin for carp after in-feed administration.According to the analytical methodology in biological samples, we developed HPLC method to detect avilamycin A in water, carp plasma and tissues and auto-biography method to determine avilamycin in tissues. Average recoveries, detection limit and relative standard deviation were studied. Calibration curves were established for measured samples, and then study the pharmacokinetics and residue depletion by animal test.Carps (Cyprinus carpio L.) were administrated avilamycin intravenously with a single dosage of 4mg/kg b.w. The plasma concentration time data were best described by two-compartment open model. The t_1/2α and t_1/2β was 0.11h and 0.8h, respectively. The V_d. AUC and the CL(?), was 0.34mL, 4.94μg/mL ? h and 0.81L/kg · h^-1, which indicated that avilamycin could be distributed rapidly and widely in carps. Following oral administration for dose up to 40 mg/kg b.w., avilamycin in carp plasma was still...