Studies On Diarrhea Of Piglets And Production Traits And Their Relationships With NRAMP1 Gene

The domestic animal disease, particularly the contagion diseases is big enemies to livestock husbandry and prodution, which restrict the developments of the livestock husbandry. The loss resulted by diseases is about 12～15% in the whole value of livestock husbandry production. Along with the improvement of intensive and the change in feeding condition, we are discovering the occurrence and fad of the new infectious disease one after another again. However, Using medicine excessively will result in the food pollution. This balance strategy that needs to develop is to reduce the usage of medicine passed by improving in resistence ability of domestic animal. Along with the quick development of the molecular biology, the perfection in livestock heredity diagram and the complementarity in inheriting marking, some genes or the heredity marking that have something to do with the disease successively discovered now. Adopts the genetics method to raise the anti-disease dint of the livestock essentially from the heredity, It is the valid path that resolves that problem, which adopts the genetics method to raise the anti-disease ability of the livestock essentially from the heredity, carry out an anti-disease breeding, sieve anti-disease system. In this research we studied the association of Polymorphisms of NRAMPl gene with diarrhea traits of Piglet and some production traits in order to analyze whether the gene could be a candidate gene for disease resistance.PCR-SSCP analysis was used to detect the variations within introns 1 of the porcine NRAMP1 gene in the same conditions which were reared in the spring and autumn of 2005, involving three breeds piglets of Landrace 794, piglets of Minpig 179, piglets of Landrace and Minpig 348, piglets of Yorkshire 258. We observed and noted the diarrhea circumstance of the piglets, carry on the observation record, compute the near hand over coefficient in Minpig, carry on analysis on the heredity factor that influences Minpig diarrhea; Carry on a measurement on production characters. Piglet born alive, litter size 7 days, litter size 14 days, litter size 21 days, litter size weaned, and the death condition. The origin studies a main result as follows:1 The experimentation first found the SNPs of introns 1 of the NRAMPl gene where polymorphism is leaded by A113G and G164T. The difference of distributing in SNPs between the rate of genotype and the frequencies of genotypes is significantly difference (P<0.01). There are few of CC genotype among these four groups, and no BC genotype be tested in Large-Yorkshire group.2 The frequencies of genotypes and alleles for NRAMP1 polymorphism locus were...