Studies On FSHR Gene Polymorphism And ADD1 Gene Cloning And Sequencing Of Goat

Goat breeding is the important part of animal husbandry, in the social background of social economic rapid development ,quality of life and level of consumption common improvement and meal structure constantly changing ,that make goat breeding is more important than before , so developing sheep farming energetically and improving the mutton genetic performance are the keystone of animals breeding task of our country .Of which, it is all along our antipant goal that reduce cost of production ,advance economic benefit and increase animal product yield through increase twinning rate of sheep, so what searching after major gene that increase litter size from hereditary constitution and gene expressive level is be regarded as important things at home and abroad.For these reasons ,we select goat follicle-stimulating hormone receptor (FSHR) gene and adipocyte determination and differentiation factor-1(ADD1) gene as reproductive traits and meat quality traits gene to study the correlation between goat FSHR gene polymorphism and litter size and the sequences characteristic of goat ADD1 gene. Material work and conclusion as follows:(1) SNPs of follicle-stimulating hormone receptor (FSHR) gene and its relationship with the litter size in 3 goat breeds① Applying the powerful PCR-SSCP technique to analyze the polymorphism of FSHR gene in 3 goat breeds, the results were showed, fragments amplified by primer had three genotypees (AA, AB and BE), mutation gene frequency in Guizhou black goat was obviously wanted to be higher than that in Xiangdong black goat and Nanjiang brown goat. X~2 fittness test showed that locis in three goat speices were in a state of Hardy-Weinberg equilibrium.② We cloned and sequenced fragments amplified of homozygosity (AA,BB), and BLAST comparision with the same sequence in GeneBank , it showed that BB has only two mutations CT→GC in 702,703bp of cDNA compare with Genebank sequence (AY765375) , AA has no such mutation but has 4 other mutations C→A,G→C, C→A,C→T in cDNA665,678, 695, 763 seperately. To synthesize two homozygosity, there has 6 mutations .According to the explain of gene submitter Ouyang xu xiang ,the bound of 598-757 is 5'-UTR and 758-860 is CDS, mutation in cDNA763 is in the CDS, We translated this mutual and normal sequences into amino acid , it showed this base mutation did not cause the change of the amino acids.