| This subject was studied the effect of different P, K concentration in nutrient by water cultivates with two cottons varieties'XinLuzao 13'and'ZhongMian36' on growth development and physiological characteristic of cotton.And the main results were as follows:1 In this experiment, high effective liquid phase chromatography (HPLC) was used for the separation and determination three kind of endogenes hormones content in cotton that is a simple,rapid and perfect method . The chromatograph condition are as follows :the mobile phase was a mixture of acetonitrile and water (volume ratio: 4:6 ), the velocity was 1ml/min with an input of 10μl,the detector wave length set at 254 nm.2 .Compared with CK, the growth of cotton was promoted obviously, and stem height , the leaf area and the leaf of stem number were all increased, and the time about the leaf of stem to speed up because of boosting of P, K concentration in nutrient properly. Under this trial conditions, a cotton high growth assumes "S" the shape curve growth is the initial period slow, intermediate stage quick, later period also is slow, until stops growing.3 Compared with CK, the growth of cotton root was promoted, and the root volume, the root total area, the activity of roots were obviously increased because of increasing P in nutrient. The sufficient K nutrition also promoted the cotton root system's growth, the activity of roots, the total absorption and active absorption area were increased, but there is discrepancy in difference varieties, low potassium surpasses high potassium and CK about'XinLuzao 13' ,'ZhongMian36' is more superior along with the K concentration increasing.4 The content of Chla, Chlb, and the chlorophyll total quantity was improved obviously because of increasing of P concentration in nutrient, So the changes avoided premature senility,and enhanced photosynthesis. Effect of K on chlorophyll was same to P, the content of Chla, Chlb and Chl were all increased with the improving of K in nutrient,but high potassium was not propitious to the synthesis of chlorophyll, two varieties cottons display consistently, the content of chlorophyll of low potassium is more than that of high potassium,which is more than that of CK in this experiment.5.The synthesis, the transportation and metabolism of endogenes indoles-3-acetic acid(IAA),zeatin(Z) and gibberellic acid (GA3) in cotton were advanced with promoting of P, K concentration in nutrient.The different varieties had different resuit in different P,K concentration,the content of IAA,Z and GA3 in low phosphorus and potassium is more than high phosphorus and potassium in'XinLuzao 13' ,but high phosphorus and potassium is more than low phosphorus and potassium in'ZhongMian36'. The endogenes indoles-3-acetic acid,zeatin and gibberellic acid in various organs distribution in all kinds of P,K level present the regularity, which is that the content of zeatin of leaf is more than that of root which is more than that of stem, the content of gibberelic of leaf is more than that of stem which is more than that of root, the content of indoles-3-acetic acid of stem is more than that of leaf which is more than that of root.6.The proper concentration of P and K in nutrient can promoted the absorption of N, P and K. The absorption accumulation of nitrogen and potassium in low level is more than other treat in'XinLuzao 13',but The absorption accumulation of phosphorus and potassium in high level is more than other treats in'ZhongMian36'.7 Significant correlation was found between the content of,Z,GA3 and IAA and the absorption accumulation of nitrogen and potassium under the phosphorus nutrition, the correlation coefficients were 0.9304**, 0.9442**, 0.9927** (with N), 0.8875*, 0.9813**, 0.9080* (with K), but it was not obvious about phosphorus . Under potassium nutrition, only between the content of, IAA and the absorption accumulation of nitrogen and potassium have the remarkable correlation, the correlation coefficient is 0.9180** and 0.9769**. |
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