Study On The Technique System Of Tissue Culture And The Character Of Photosynthesis Of Oxalis Vidacea L.

Oxalis vidacea L. is one of very high value group cover plants and it′s leaves are very beautiful. Oxalis vidacea L. doesn′t need to be conserved carefully and is not easy to be infected by plant diseases and insect pests.It is used for afforestation in garden.When the temperature is less than 5℃,the leaves and the leafstalks would die.At present,the main propagation method is the way by bulb.Because the propagation coefficient is not high,the price of Oxalis vida-cea L. is also high.These factors limit its use.The article researched several as-pects including phenology,the character of photosynthesis and tissue culture in order to use the plant elasticly.In order to incease the propagation rate and realize industrialization,mastering technology of tissue culture is essential.This experimental place is Hefei city.And the result of this experiment as follows:1. The growing period of the plant is from March to December and the flower season is from April to November.The leaves and leafstalks would die When the temperature is less than 5℃or having a heavy frost.Strong light is situable for the plant . But when it sprounts,umbrageous condition is essential.Oxalis vidacea L. loves warm and moist environment. The depth of plant ougnt to less than 20cm in clay and it fits for moderate prune2.This tissue culture experiment adopts way of organ and the best explant is leafstalk.The best effective flow of asepsis method is as following:explant→clean up by tap water→dip in the 70% alcohol about 10s→dip in the 0.1%Hgcl2about 5min→wash 6 times by asepsis water→inoculate.Basic culture medium is MS and the main hormone are BA and NAA. The test of three factors is best in contrast to the test of one factor and two factors.Specific result as follows: MS+2,4-D0.6mg/L+NAA0.4mg/L+BA1.0mg/L +sugar25g/L+agar7g/L is used for inducement. The best multiplication medium is MS+2,4-D0.2mg/L+NAA0.4mg/L+BA1.5mg/L+sugar25g/L+agar7g/L. MS+2,4-D0.2mg/L+NAA0.4mg/L +BA1.0mg/L +sugar25g/L+agar7g/L is suitable fou inducing root and the best medium for bulb is MS+2,4-D0.2mg/L+NAA0.4mg/L +BA0.5mg/L +sugar25g/L+agar7g/L.The condition of tissue culture is listed as belowing:light strength is 2000 lx,the time of light is 12h in a day and pH is 5.8.One cycle which is from inoculating to transplanting needs half and three months.The combination of active charcoal has active function but the effect is not notable.When transplanting,normal cultivate medium is good.If the temperature is between 20℃and 30℃,the survival rate could reach 100%.3.In summer net photosynthetic rate of Oxalis vidacea L. is highest.The order of average net photosynthetic rate as follows:summer is greater than spring and spring is greater than autumn.The most important factor is air temperature in spring.The main factors are intercellular CO2 concentration and percent of water in summer. Intercellular CO2 concentration and illumination intensity are the two important factors in autumn.The correlation between net photosynthetic rate and intercellular co2 concentration is contrary.The compensation point is about 20μmol·m-2·s-1 and the saturation point is 1300μmol·m-2·s-1 in spring. The compensation point is about 45μmol·m-2·s-1 and the saturation point is 900μmol·m-2·s-1 in summer. The compensation point and the saturation point are 80μ·molm-2·s-1 and 600μmol·m-2·s-1.4. The Water Use Efficiency of Oxalis vidacea L is lower .This shows it′s drought-resistant ability is weaker.The WUE in summer is highest and the WUE in spring is higher than in autumn.The average value of WUE in autumn is negative.