Authentication And Analysis Genetic Effect Of Ae Gene In Zea Mays L.

Maize is one of the most important crop plants in the world and cornstarch is a main starch source of human being. The starch is a main accumulated material of the corn seed, and starch weight is about 70% of the seed. The starch exists in two forms: amylopectin and amylose. The common cornstarch is a mixture of 28% amylose and 72% amylopectin respectively. Because of special physical and chemical characters, Amylose starch is a kind of high additional value starch because of special physical and chemical characters. Also,amylose starch is an important industrial material and extensively applied in more than 30 fields.However, the amylose content in common corn is rather low, generally between 22% and 28%.This limited amylose application in industry because the cost of separating the high amylose from the common corn is very high. Thus, it is necessary for corn breeders to explore high amylase corn to fit for industrial use. The breakthrough of high amylose research is the discovery of the ae (amylase extender) gene mutation body. The amylose contents will increase if maize seed including ae gene. Afterwards other modifying genes were discovered which affect the ratio of amylopectin and amylose.In this paper, 22 pairs of specific primers were designed according to the ae gene sequence published in Genbank. Fourteen inbred lines of common corn, four ae mutant inbred lines, and sixteen materials of hybrid lines were marked. Also, 1000 grains seed weight and amylase contents of 34 varities of maize were tested. The results as following:The amylose content of ae hybrid lines (F₂) are improved, the range is 29.43⁴8.2. But it is difficulty to obtain high amylose which amylose content exceeded to 70% only by recurrent breeding. So it is necessary to develop recurrent selection for the goal of high amylose content maize.Compared with hybrid lines (F₂), the ae inbred lines of 1000 grain weight were descended, which ranged from 0.4% to 38.4%. The main reason was the satiety of ae hybrid lines (F₂) seeds was declined. The best combination is chunyu1♀/ aew23, wnich...