Selection Of The Main Candidate Genes Which Affect Meat Traits Of Mongolia Bovine

In order to investigate the main candidate genes which affect meat traits of Mongolia bovine, we studied Mongolian cattle gluteal muscle tissue from two developmental stages (16 months and 5 years old) by using mRNA differential display PCR. And additionally, we researched the differential expression of these genes on the different developmental stages of Mongolia bovine muscle tissue through relative quantitative PCR.Six differentially expressed fragments were selected by using mRNA differential display PCR. Compared the fragments sequences with BLAST after sequencing, the result show that one of them was Mongolia bovine's cofilin2 gene, the others have no similar gene sequences. Cofilin2 gene codes cofilin2 protein which is the most potent regulator of actin in muscle tissue. The selective expression of this gene can cause beef tenderness changes. Therefore, cofilin2 gene can be deemed as one of the main candidate genes which affect meat traits of Mongolia bovine.Furthermore, we used relative quantitative PCR to identify the reality of the differential expression gene cofilin2 and to detect its differential expression. The results showed that the expression of cofilin2 in 5 years old Mongolia bovine's muscle tissue was 3.8 times more than in 16 months old ones. Excessive cofilin2 can inhibit G-actin polymerization and accumulation of type 1 muscle fiber, therefore, the high expression of cofilin2 gene may cause quality decline of the meat traits of Mongolia bovine.The successful acquisition of cofilin2 gene and the research of its differential expression provide important theoretical and experimental evidences for nurturing excellent Mongolia bovine and producing high quality and green healthy beef.