| The goose plague, caused by Goose Parvovirus (GPV) in gosling and Muscovy duckling within 30 days old, is an epidemic contagious infection with diarrhea, embolism in small intestine and high mortality. It's necessary to establish a simple and rapid diagnostic method of the disease for outbreak cases are often infected contemporaneously with muscovy ducks parvovirus and difficult to diagnose by epidemiology, clinical symptoms and postmortem. In this study, direct immunofluorescence assay (DFA) was developed to detect GPV antigen, with dialysis of GPV monoclonal antibody labeled by fluorescein isothiocyanate (FITC). The results showed that positive reaction to GPV is only observed after DFA reacts to MDRV, MPV and PMV for 60 minutes. Optimum concentration of the reagent of DFA is 1:150, it is also sensitive until dilution by 1:450. Compared to pathogen isolation and indirect immunofluorescence assay (IFA ), coincident rate between them was 100%, was 95%, respectively and average coincident rate of DFA, IFA, and pathogen isolation was 95%. With DFA, the distributions of the virus in various tissues were determined at the third day, the 5th day, the 7th day and 10th day after muscovy ducklings were challenged by GPV,showing that GPV is detected obviously from heart, liver, spleen, kidney, lung, pancreas and caecum at the early stages of infection and the virus is detected obviously from pancreas and caecum at the later stages of infection. Sensitivity of DFA to GPV was further confirmed. The results indicate that DFA is specific, sensitive, repeatabe to determine GPV, it can be used for the rapid diagnosis and epidemiological investigation of Muscovy duckling infection of GPV.
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