The Relationship Between The Food Of Sika Deer And The Fragment Of Tissue In The Excrement

It evaluates the relationship between fragment of tissue and feed intake by the method of scatology analysis. It imitates the way of herbivore eating in the wild from August to September 2010. The experiment uses 21 species of plants to feed sika deers in deer center. We collected 17 samples of excrement. The excrement use 3 methods to process that are concen-trated nitric acid, dissociation buffer and Hertwing. It uses 3 methods to microscopic examination that are frequency conversion, direct count and sample method.The results showed that the method of scatology analysis can indicate the composition of herbivore food habits accurately. All the 21 species of plants can be found in the excrement. There are no significant difference between actual data and estimated datum in quantitative analysis for food (P>0.05). The estimated datum came from 3 kinds of microscopy methods. But all the estimated datum came from 3 microscopy methods have errors. The datas from sample method have the leasterror (ED=26.734). The error came from direct count is maximum (ED=34.23). The error from frequency conversion is middle (ED=28.623). If the pro-portion of some plants is under ten percent, they are underestimate. On the contrary if the proportion of some plants is higher than fifty percent, they are overestimate. The best effect in the three processing method is concentrated nitric acid, the worst effect is Hertwing, and the effect of dissociation buffer is middle. There are no direct relationship between the dissociation buffer of plants and the size of plants fragment. The identification proportion and the size of plants fragment have no direct relationship. Experiments prove that scatology analysis can use in the quantitative analysis when we analyse the food of herbivore. The scatology analysis can provide trophic analysis with basis.