Optimized Production Mode And Established Technical System For Industrialized Breeding Of Taxue Cuspidata

With the rapid development of modern agriculture, industrialization breeding has become a proven advanced agricultural technology. Taxue Cuspidata as materials of rare medicinal herbs and the Greening, which resources is shortage. And the industrialization seedling of Taxue Cuspidata is developing slowly. Therefore, to resolve the resource shortage of Taxue Cuspidata, it demands shorten the seedling age for saving seedling time, improve seedling production efficiency for reducing costs, promote new technologies so that the annual continuous production of industrial seedling production. This work is supported by Changchun Scientific and Technological Support Projects"Industrialization seedling key technologies of Tissue Culture in Taxue Cuspidata"(2009KZ22). In this paper, we describe the propagation technology of Taxus cuspidata via open and simplified tissue culture, microcuttage and Hydroponics method of in vitro plantletsThe main contents and conclusions in this paper are as follows:1.Open and simplified tissue culture of Taxus cuspidata, through adding different concentrations of potassium sorbate (0, 0.02, 0.04, 0.06 , 0.08 and 0.10g/L), chlorothalonil (0, 0.5, 1, 1.5, 2 and 2.5g/L), green tea extract (0, 3, 6, 9, 12 and 15g/L), streptomycin (0, 0.2, 0.3, 0.4, 0.5 and 0.6g/L) to culture medium instead of high pressure sterilization, observing the antimicrobials agents on the antibacterial activity in culture and the effect of Taxue Cuspidata explants induction rate and the growth situation, to screen out the optimal antibacterial agent used in open tissue culture to replace the high-pressure sterilization. Test to obtain the optimal antimicrobial agent is agricultural streptomycin; the optimal concentration is 0.4g/L.2. Test of open and simplified tissue culture of Taxus cuspidata. Studying the influence of various concentrations of the basic components in B5,which are carbon source, a large number of elements, inositol and coagulants,on inductivity,callus production time and growth situation of explants. Test showed that, there was no obvious differences in growth of plantlets in vitro of phalaenopsis using sugar to replace sucrose and reduced a large number of elements to 1/4.Using carrageenan which is half of agar powder in the basic B5 medium replaced with agar powder can enhance the callus grow. And to the contrary, inositol reduction the amount of processing is not conducive to the proliferation of the explants.3. Test of Simplify the culture vessel. Contrasting the use of plastic cups + plastic sealing membranes , erlenmeyer flask + plastic sealing membranes and erlenmeyer flask + cotton plug to the influences of explants induction, The results showed that the plastic cups + plastic sealing membranes is the optimal ,which not only can reduce costs, the most important is the Taxus cuspidata explants had no effect on induction and growth.4.Micro-Cutting Propagation technology of in vitro plantlets of Taxus cuspidata with semi-woody stem (10~15cm) had been studied the influence of different concentration rooting agents and substrates. In single factor test,indole butyric acid (IBA), indole acetic acid (IAA) and naphthylacetate (NAA)(50, 100, 150, 200 and 250mg/L) in five levels of three rooting agents, the test shows that indole butyric acid (IBA) make the highest rooting rate, with an average of above 80%;Indole acetic acid (IAA) makes the most root length, using a concentration of 150mg/L, the root length to 10.4cm; Naphthylacetate (NAA) makes the root number grow stable, the average root number is above eight. According to the results of single factor test to select the suitable concentration of rooting reagents orthogonal test, the results showed that the three rooting agent interaction for optimal combination is Naphthylacetate 250mg/L, indole acetic acid 250mg/L and IBA 150mg/L .5. Screening micro-cutting test by Simplex centroid design combined garden soil (z1), sand (z2), sawdust (z3) and slag (z4) to 15 kinds of formula and studied the conditions on the growth of cuttings. Then, get equations. The principal factor method were applied to analyze the model of matrix components , tested and analysis the soil density , soil volume weight, soil porosity and soil moisture content. Then, optimized the quality of the formula got the best formula is the garden soil 62.07% and sand 37.93%.6. Controlled parameters of cultivation environment in cuttings hydroponics, based on the shoot growth under hydroponic, from the training support materials (sponge and foam board), electrical conductivity (2.5, 3.0, 3.5, 4.0 and 4.5mS/cm), pH (5.0, 5.5, 6.0, 6.5 and 7.0), ventilation (0, 0.05, 0.10, 0.15 and 0.20vvm) optimal parameters. Tests showed that the best thing for the hydroponic support is foam, the optimum nutrient solution conductivity is 2.5mS/cm, the optimum pH is 6.0, and the optimal ventilation is 0.10vvm.In this paper, the three key technologies of seedling models were analysis, and established the cultivation technology system. Application of fuzzy mathematics method to comprehensive evaluation the three kinds of seedling models, the result shows that open and simplified tissue culture of Taxus cuspidata is the most favorable seedling method, it has the features of saving material, propagating faster and economical, the cost of plantlets is 0.1007 Yuan per plant; Micro-cutting do not need complicated technology and facilities, it suit for small scale, low initial investment and relatively extensive production; Hydroponics propagation of Taxus cuspidata callus culture got parameters of the cultivate environment, to get rooting parameters need further study. This study shows the rapid propagation of Taxus cuspidata seedlings in the industrial seed culture's potential, make a good foundation in further study about propagation of Taxus cuspidate.