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Study On The Effect Of The Newcastle Disease Oil Emulsion Inactived Vaccine Astragalus Polysaccharides Houhai Acupoint Injection Of Immunity Function In AA~+ Broilers

Posted on:2012-10-09Degree:MasterType:Thesis
Country:ChinaCandidate:Y C GuoFull Text:PDF
GTID:2143330335489219Subject:Clinical Veterinary Medicine
Abstract/Summary:PDF Full Text Request
In this study, Astragalus Polysaccharide Injection with Newcastle disease oil emulsion vaccine as experimental material, through the houhaixue injection Expand on Chinese immunoenhancer and a new immune enhancer Immune Ways.One old day age will be 350 AA+ flesh chickens were randomly divided into seven groups by weight, the firstâ… group as control group, subcutaneous injection vaccine 0.4mL, The firstâ…¡group subcutaneous injection vaccine 0.2mL+astragalus polysaccharides 0.2mL, The firstâ…¢andâ…¢g roup were houhaixuevaccine 0.4mL and 0.2mL, vaccine The firstâ…¤,â…¤andâ…¤group were houhai vaccinexue 0.2 mL+astragalus polysaccharides 0.2 mL, vaccines 0.1mL+astragalus polysaccharides 0.3mL and vaccine 0.05mL+astragalus polysaccharides 0.35 mL, for 42 days. Respectively on 14, 21, 28, 35, 42 day-old randomly every group, after ten weighing profile kill determination the Immune organs,Cellular immunity, humoral immune, Immune molecules ,the results showed that:Immune organs :immune organs indexâ…¤group significantly higher thanâ…¡group andâ…¤group (P<0.05), Immune organ development ibdvinfectedâ…¤group significantly higher thanâ…¤group (P<0.05).Cellular immunity :the transformation of lymphocyteâ…¤group significantly higher thanâ…¤group andâ…¤group (P<0.05), Soluble content determination of CD4, CD8â…¤group significantly higher than other groups (P<0.01).Humoral immune : NDHI antibody titerâ…¤group significantly higher thanâ…¡group,â…¢group,â…¢group,â…¤group andâ…¤group (P<0.05). Immune globulin IgA, IgM, IgGcontentâ…¤group significantly higher than contentâ…¢group andâ…¤group (P<0.05), Total protein, albumin, globulin contentâ…¤group significantly higher thanâ… group,â…¢group andâ…¤group (P<0.05).Immune molecules :interferon - gamma concentrationâ…¤group significantly higher thanâ…¢group,â…¤group andâ…¤group (P<0.05). IL-2 concentrationâ…¤group significantly higher thanâ…¡group,â…¢group andâ…¤group (P<0.05).Conclusion: 1. Chicken Newcastle diseas oil emulsion inactivated seedlings astragalus polysaccharides houhai acupuncture point injection can improve immune organs index, boost immune organ development.2. Chicken Newcastle diseas oil emulsion inactivated seedlings astragalus polysaccharides houhai acupuncture point injection can significantly improve the cellular immune, humoral immune , immune molecules function.3. Throughout the test results, Houhaixue vaccine 0.2mL+astragalus polysaccharides 0.2 mL can significantly improve the immune function of AA + flesh chickens, suitable for clinical practice.
Keywords/Search Tags:Houhaixue Injection, Chieken Newcastle diseas, Astragals Polysaeeharid, imxnunoPoteniiato, Immune Function
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