| In the present study, two related F8:9 recombinant inbred line (RIL) populations were generated to localise genetic factors controlling seven quality traits: grain protein content(GPC), wet gluten content (WGC), flour whiteness (FW), kernel hardness (KH), water absorption (Abs), dough development time (DDT) and dough stability time (DST). These lines were derived by crossing Weimai 8 and Jimai 20 (WJ) and by crossing Weimai 8 and Yannong 19 (WY). In total, WJ comprised 485 lines, while WY comprised 229 lines. Data on these seven quality traits were collected from each line in five different environments. QTL analyses were performed using the software of ICIMapping 3.0. QTL for the seven traits were analyzed. And that high molecular weight glutenin subunits (HMW-GS) were analyzed by sodium dodecyl sulphate polyacrylamide-gel electrophoresi(sSDS-PAGE) using the two RILs. The results were as the following:1.HMW-GS were analyzed by SDS-PAGE using the two RILs, the results showed that up to 12 kinds of allelic variations in HMW-GS encoded by Glu-1 locus were detected in WJ population and 13 kinds were detected in WY, totally twenty-three combinations were observed among the variations, of which 2 were uniform with the two parents and 21 were new types in WJ, twenty-four combinations were cbserved in WY.2.We tested the polymorphism of 3123 available markers between the parental varieties. Of these, 256 markers revealed polymorphism between the parents of the WJ population and 237 markers revealed polymorphism in WY. The result showed that both mapping populations were suitable for map construction.3.The genetic map constructed based on the WJ population included 344 loci distributed over all wheat chromosomes and spanned 2855.5 cM, with average density of one marker per 8.30 cM. There were six linkage gaps with distances > 50 cM. Marker distribution ranged from 45 on chromosome 4A to 3 on chromosomes 4D and 7D. The WY population was used to establish a genetic map consisting of 358 loci distributed in 27 linkage groups because of six linkage gaps. The map covered 3010.70 cM of the whole genome with an average distance of 8.41 cM between the adjacent loci. The number of markers per chromosome ranged from 40 on chromosome 1B to 3 on chromosomes 3D.4.Based on the two genetic maps established from the RIL populations, the QTL were detected using the software ICIMapping. QTL for 7 quality traits were analyzed in 6 environments. A total of 150 QTL were detected and distributed on 21 chromosomes in the two populations. Among them, 3 QTL were major genes in WJ, while 25 QTL were major genes in WY. A major QTL for GPC, accounting for 53.04% of the phenotypic variation, was detected on chromosome 5A. A major QTL for WGC also shared this interval (Xcfa2163.2-Xcwm216), explained more than 36% of the phenotypic variation, and was significant in two environments. At least thirteen pairwise were common to both populations, accounting for 20.00% and 15.29% of the total QTL in WJ and WY, respectively. |
