Study On Genetic Diversity Of Nigeria African Honeybee, Local Apis Mellifera And Theirs Hybrid Generation â…¡ With Morphometric Characters And Microsatellite DNA

10 main indexes of external morphology of AnHui Local Apis mellifera, Nigeria African honeybee and theirs hybrid generationⅡwith each 50 worker bees as the research material were mensurated, and 15 microsatellite makers were used to analyzed the genetic diversity of the three populations for analysing theirs genetic diversity with methods of morphology and microsatellite DNA polymorphism.The main results were summarized as follows.1. It had extremely significant differences(P<0.01) in tongue length, length of bee right forewing, area of the anterior wing, length of bee right forewing cubital vein a, length of tergum 3, length of tergum 4 and length of tergum 3+4, and significant difference(P<0.05) in width of bee bee right forewing, but no significant differences(P>0.05) in length of bee right forewing cubital vein b and index number of cubital vein Ci.2. 15 pairs of microsatellite primers were tested in AnHui Local Apis mellifera, Nigeria African honeybee and theirs Hybrid generationⅡand could be successfully amplified. The results showed that 121 alleles were found in 15 microsatellite loci, mean number of effective alleles was 8.067, the number of alleles per locus ranged from 3 to 13, and indicated that rich genetic diversity in the three populations.3. The expected heterozygosity(He) of all populations in 15 microsatellite loci ranged from 0.5801 to 0.8526, and the average value was 0.7591±0.0762. The polymorphism information content(PIC) ranged from 0.5158 to 0.8363,and the average value was 0.7283±0.0879.The average expected heterozygosity of Local Apis mellifera, Nigeria African honeybee and theirs hybrid generationⅡwas 0.6208, 0.5780, 0.7451, respectively, and showed high heterozygosity and rich polymorphism.4. In this study, the genetic differention between Local Apis mellifera and Nigeria African honeybee measured as FST value was 4.36%(P<0.01), and 8 loci were contributed significantly( P<0.01) to this differention, and measured as FIS value was 0.070(P<0.05), all the loci were contributed significantly(P<0.01) to this differention, that indicated less inbreeding between the two populations.5. The Reynold's distance values of Nigeria African honeybee and Local Apis mellifera was 0.725, and the Nm value was 0.213, showed further Reynold's distance and less gene flow. Local Apis mellifera was belong to Europe type Apis mellifera Linnaeus Apis, and African honeybee is African type. They could not have normal crossbreeding, and showed less communication.