Increasing Density Of Wheat Genetic Linkage Map And QTL Analysis For Soluble Saccharides Contents In Grains Of Wheat

A high-density genetic map is indispensable for wheat genetic research. Microsatellite DNA markers were widely used in genetic linkage map of wheat, because of its merits of simple manipulation, good stability, codominant and high polymorphism. Chromosomes with less molecular markers on the wheat molecular genetic map which already has been constructed were counted. SSR markers from GrainGenes were found online. The polymorphism of primer pairs were detected using recombinant inbred lines (RILs) and their parents, and the marker loci were mapped using RIL population and Mapmaker/Exp3.0 software.The component and contents of soluble osaccharides in wheat were studied by high performance liquid romatography (HPLC). QTLs of soluble osaccharides contents were mapped using the RILs of wheat. The main results were as follows:(1) A total of 461 SSR pairs from GrainGenes, including Xcfa,Xcfd,Xbarc,Xgwm and Xwmc, were found online. The polymorphism of 123 SSR pairs, 78 Xbarc pairs, 9 Xgwm pairs and 2 Xwmc pairs, were detected using the parents of RILs.(2) Using our RIL population, the polymorphism of 26 SSR primer pairs with 34 loci was detected, and 23 of those loci were integrated on 11 chromosomes on our wheat genetic map, 1B,2A,2D,3A,3B,5A,5B,6A,6B,6D and 7A.(3) A total of five soluble osaccharides, Fructose, Sucrose, Maltose, and Raffinose, were found in grains of wheat by high performance liquid romatography (HPLC).(4) Using the recombinant inbred line population, thirteen additive QTLs for soluble oligosaccharides contents were detected on 11 chromosomes, 1B, 1D,2B,2D,3B,4A,5A,5D,6B and 7B, with a single QTL explaining 4.97%-53.57% of phenotypic variations.